灵芝提取物中灵芝多糖质量控制方法研究  

Study on the quality control of Ganoderma lucidum polysaccharides from Ganoderma lucidum extracts

作　　者：王艳莉 张国兴 WANG Yan-Li;ZHANG Guo-Xing(Beijing Tongrentang Healthcare Pharmaceutical Co.,Ltd.,Beijing 100176,China)

机构地区：[1]北京同仁堂健康药业股份有限公司,北京100176

出　　处：《食品安全质量检测学报》2025年第5期155-161,共7页Journal of Food Safety and Quality

摘　　要：目的 建立灵芝提取物专属性鉴别方法,实现多种单糖同步测定。方法 采用柱前衍生化反相液相色谱分析法,分析灵芝提取物中单糖组成种类,建立一测多评方法测定灵芝提取物中单糖含量结果。结果 3个厂家的灵芝提取物含有甘露糖、葡萄糖、半乳糖,但有4批次各单糖间比例相差较大。一测多评经方法考察可以同时测定甘露糖、葡萄糖、半乳糖含量,与外标法相对偏差均小于5%,无显著性差异,具有可行性。结论 本研究通过分析灵芝提取物中单糖组成及单糖间比例,可作为鉴别灵芝提取物中灵芝多糖质量的辅助手段,为企业在监控提取物质量提供参考思路。Objective To establish a specific identification method for Ganoderma lucidum extract,and achieve the synchronous determination of a variety of monosaccharides.Methods The analysis of monosaccharide composition in Ganoderma lucidum extracts was conducted using a pre-column derivatization reversed-phase liquid chromatography method.A quantitative analysis of multi-components by single-marker method was established to determine the monosaccharide content in Ganoderma lucidum extracts.Results Ganoderma lucidum extract from 3 manufacturers contained mannose,glucose and galactose,but the proportion of monosaccharides in 4 batches was significantly different.The content of mannose,glucose and galactose could be determined by one test and multiple evaluation method at the same time.The relative deviation from external standard method was less than 5%,and there was no significant difference,which was feasible.Conclusion This study analyzes the monosaccharide composition and monosaccharide ratio in Ganoderma lucidum extract,which can serve as an auxiliary means for identifying the quality of Ganoderma lucidum polysaccharides in Ganoderma lucidum extract.It provides a reference for enterprises to monitor the quality of extracts.

关 键 词：灵芝多糖 质量控制 单糖组成 一测多评 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]