化州柚叶绿体基因组及其结构分析  

Analysis of chloroplast genome and structure of Citrus grandis‘Tomentosa’

作　　者：黄琼林 陈依虹 吴民富 叶晓霞 谭靖怡 吴民华 HUANG Qionglin;CHEN Yihong;WU Minfu;YE Xiaoxia;TAN Jingyi;WU Minhua(School of Basic Medicine,Guangdong Medical University,Zhanjiang,Guangdong,524023,China;Department of Food Science,Foshan Polytechnic,Foshan,Guangdong,528137,China)

机构地区：[1]广东医科大学基础医学院,广东湛江524023 [2]佛山职业技术学院食品科学系,广东佛山528137

出　　处：《中国南方果树》2025年第1期1-8,共8页South China Fruits

基　　金：广东省基础与应用基础研究基金(2020A1515110332);广东省大学生创新创业项目(S202310571113);广东医科大学大学生创新创业项目(GDMU2022113)资助。

摘　　要：采用高通量测序技术对化州柚Citrus grandis‘Tomentosa’叶绿体基因组进行测序,使用生物信息学方法对其进行组装、注释以及解析。结果显示,化州柚叶绿体基因组为160139 bp的环状双链四分体,由87733 bp的大单拷贝区、18410 bp的小单拷贝区以及穿插两者之间的两个26998 bp的反向重复区构成,含有133个基因。化州柚叶绿体基因组共有26244个密码子,其中以A/T碱基结尾的密码子居多;在检测到的104个简单重复序列(SSR)中,以A/T碱基的单核苷酸重复基序为主。化州柚等柑橘属植物叶绿体基因组的单拷贝区/反向重复区边界构成和全序列相似度较高,变异碱基主要分布在trnS-trnG、petN-psbM、psbC-trnS、trnT-trnL、accd-psaI、petA-psbJ、rps3-rpl22、ndhF-rpl32、ccsA-psaC和ycf1等基因间隔区或基因。系统进化分析显示化州柚与同属植物柚、甜橙的叶绿体基因组同源性最高。The whole chloroplast genome of Citrus grandis‘Tomentosa’was sequenced using high-throughput sequencing technology,and was assembled,noted and analyzed with bioinformatics approaches.The results demonstrated that the C.grandis‘Tomentosa’genome had a circular double-strand tetrad structure with a size of 160,139 bp,including 87,733 bp of large single-copy region,18,410 bp of small single-copy region,and two 26,998 bp of inverted repeat regions,containing 133 genes.There were 26,244 codons in the C.grandis‘Tomentosa’chloroplast genome,most of which ended with A/T bases.Among the 104 detected simple sequence repeats(SSRs),the predominant motif was the mononucleotide repeat motif of A/T bases.The boundary composition and overall sequence similarity of the single-copy region/inverted repeat region of the chloroplast genome in Citrus plants such as Huazhou pomelo were relatively high.The variant bases were mainly distributed in the intergenic regions or genes such as trnS-trnG,petN-psbM,psbC-trnS,trnT-trnL,accd-psaI,petA-psbJ,rps3-rpl22,ndhF-rpl32,ccsA-psaC and ycf1.Phylogenetic analysis indicated that the chloroplast genome of C.grandis‘Tomentosa’had the highest homology with the chloroplast genomes of pomelos and sweet oranges.

关 键 词：化州柚 叶绿体基因组 比较分析 系统发育分析 

分 类 号：S666.3[农业科学—果树学]