基于UPLC-Q-Orbitrap-MS分析芪苈强心胶囊的物质基础及其体内分布  

Material Basis and Its Distribution in vivo of Qili Qiangxin Capsules Analyzed by UPLC-Q-Orbitrap-MS

作　　者：张建伟[1] 华杰凯 李荣胜 王琴[1] 常昕楠[1] 刘伟[1] 沈杰 ZHANG Jianwei;HUA Jiekai;LI Rongsheng;WANG Qin;CHANG Xinnan;LIU Wei;SHEN Jie(Third Grade Laboratory of Traditional Chinese Medicine(TCM)Preparations,National Administration of TCM,Shuguang Hospital Affiliated to Shanghai University of TCM,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院,国家中医药管理局中药制剂三级实验室,上海201203

出　　处：《中国实验方剂学杂志》2025年第5期185-193,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(82104616);上海中医药大学有组织科研重点专项(2023YZZ02);中国科协青年人才托举工程项目(2020QNRC001);上海市教委和上海市教育发展基金“晨光计划”项目(20CG50);上海中医药大学附属曙光医院四明基金项目(SGKJ-201711)。

摘　　要：目的:基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(UPLC-Q-Orbitrap-MS),定性分析芪苈强心胶囊中的化学成分及其体内分布。方法:采用UPLC-Q-Orbitrap-MS对芪苈强心胶囊供试品溶液、灌胃给药后小鼠的血清及脑、心脏、肺脏、脾脏、肝脏、肾脏中的化学成分进行检测,通过Thermo Xcalibur 2.2软件构建化合物信息数据库,并拟合准分子离子对应的化合物分子式,以保留时间、精确相对分子质量、碎片离子等信息,结合对照品和文献数据,鉴定芪苈强心胶囊中化学成分及其体内分布情况。结果:从芪苈强心胶囊中共鉴定或推测出233种化合物,包括萜类70种、黄酮类60种、有机酸类23种、生物碱类17种、甾类20种、香豆素类7种、其他类36种,其中73种化合物通过对照品鉴定。组织分布结果显示,分别在血、脑、心脏、肺脏、脾脏、肝脏及肾脏中检测到移行成分71、17、38、33、32、58、43种。奎宁酸、苯甲酰新乌头原碱、苯甲酰乌头原碱等37种化学成分移行入血和心脏;毛蕊异黄酮、美迪紫檀烷、芒柄花素、泽泻醇B和泽泻醇A等14种化学成分在血和各组织中均有分布。结论:该研究较全面地分析芪苈强心胶囊的物质基础及其体内分布情况,其中黄芪甲苷、丹酚酸B、人参皂苷Rb1、人参皂苷Rb3、人参皂苷Rd、人参皂苷Rg3、毛蕊异黄酮苷和芥子碱等成分可能是治疗心力衰竭的重要成分,可为芪苈强心胶囊的质量控制和药效物质基础研究提供依据。Objective:Based on ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap-MS),the chemical constituents of Qili Qiangxin capsules was identified,and their distribution in vivo was analyzed.Methods:UPLC-Q-Orbitrap-MS was used to detect the sample solution of Qili Qiangxin capsules,as well as the serum,brain,heart,lung,spleen,liver and kidney tissues of mice after oral administration.Using the Thermo Xcalibur 2.2 software,the compound information database was constructed,and the molecular formulas of compounds corresponding to the quasi-molecular ions were fitted.Based on the information of retention time,accurate relative molecular mass and fragments,the compounds and their distribution in vivo were analyzed by comparing with the data of reference substances and literature.Results:A total of 233 compounds,including 70 terpenoids,60 flavonoids,23 organic acids,17 alkaloids,20 steroids,7 coumarins and 36 others,were identified or predicted from Qili Qiangxin capsules,73 of which were identified matching with standard substances.Tissue distribution results showed that 71,17,38,33,32,58 and 43 migrating components were detected in blood,brain,heart,lung,spleen,liver and kidney,respectively.Thirty-seven components were absorbed into the blood and heart,including quinic acid,benzoylaconitine benzoylmesaconine and so on.Fourteen components were absorbed into the blood and six tissues,including calycosin,methylnissolin,formononetin,alisol B,alisol A and so on.Conclusion:This study comprehensively analyzes the chemical components of Qili Qiangxin capsules and their distribution in vivo.Among them,astragalosideⅣ,salvianolic acid B,ginsenoside Rb1,ginsenoside Rb3,ginsenoside Rd,ginsenoside Rg3,calycosin-7-glucoside,and sinapine may be the important components for the treatment of heart failure,which can provide useful reference for its quality control and research on pharmacodynamic material basis.

关 键 词：芪苈强心胶囊 化学成分分析 成分归属 超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(UPLC-QOrbitrap-MS) 组织分布 物质基础 心力衰竭 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学] G353.11[文化科学—情报学] O657[理学—分析化学]