Antigenicity and genetic properties of an Eurasian avian-like H1N1 swine influenza virus in Jiangsu Province,China  

作　　者：Fang He Huiyan Yu Liqi Liu Xiyan Li Yadong Xing Lei Yang Pengfei Yang Liguo Zhu Zi Li 

机构地区：[1]Huai’an Center for Disease Control and Prevention,Huai’an 223299,China [2]Department of Acute Infectious Disease Control and Prevention,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing210008,China [3]National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,102206,China [4]Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing,210008,China

出　　处：《Biosafety and Health》2024年第6期319-326,共8页生物安全与健康(英文)

基　　金：supported by the National Key Research and Development Program of China(Grant number 2023YFC2606500);Huai'an City Innovation Service Capacity Building Program(Key Laboratory Construction)(Grant number HAP201906);Jiangsu Province 333 Project.

摘　　要：Pigs are vital genetic mixing vessels for human and avian influenza viruses because their tracheal epitheliums possess both sialic acidα-2,6-Gal andα-2,3-Gal receptors.Cross-species transmission of influenza A viruses from swine to humans occurs occasionally.The first case of human infection with the Eurasian avian-like H1N1 swine influenza virus(EAH1N1 SIVs)genotype G4 was detected in Jiangsu Province,China,in February 2023,and backtracking epidemiological investigations did not reveal a clear source of the infection.The hemagglutination(HA)and neuraminidase(NA)amino acid variant sites,antiviral drug susceptibility,and antigenic variation of the isolated A/Jiangsu/27271/2023(JS/27271/23)virus were analyzed,and we evaluated the protective effect of sera collected from occupationally exposed populations in 2024 against the virus.Compared with the vaccine strain,the nucleotide sequence similarities of JS/27271/23 HA and NA were 96.5%and 95.2%,respectively.JS/27271/23 was sensitive to polymerase inhibitors(favipiravir and baloxavir),and the antigenicity of its HA protein was 8-fold different from that of the vaccine strain.The percentage of occupationally exposed population with antibody titers of≥40 against A/Hunan/42443/2015(HN/42443/15)and A/Jiangsu/1/2011(JS/1/11)were 7.25%and 2.25%,respectively,and the geometric mean titers(GMT)were 6.24 and 5.34,respectively.Out of 400 serum samples examined,none had antibody titers of≥40 against JS/27271/23.This suggests that low serum levels of antibodies to EAH1N1 SIVs in occupationally exposed populations may not provide adequate protection because of significant differences in amino acid sites and antigenicity between this virus and the current vaccine strain of EAH1N1 SIVs.There is no evidence of human-to-human transmission of EAH1N1 SIVs.Therefore,surveillance for EAH1N1 SIVs and the development of new vaccine strains are required.

关 键 词：Eurasian avian-like H1N1 swine influenza virus(EAH1N1 SIVs) Amino-acid sites ANTIGENICITY Drug resistance 

分 类 号：R373.13[医药卫生—病原生物学]