白花泡桐叶石油醚部位的GC-MS指纹图谱  

GC-MS Fingerprint of Petroleum Ether Fraction in Paulownia fortunei Leaves

作　　者：李雨虹 郭洪伟 黄佳 阳丽丽 李明[3] 龙国清 魏华[3,4,5] LI Yuhong;GUO Hongwei;HUANG Jia;YANG Lili;LI Ming;LONG Guoqing;WEI Hua(College of Biology and Environmental Science,Jishou University,Jishou 416000,Hunan China;Xiangxi Natural Biotechnology Co.,LTD,Jishou 416000,Hunan China;School of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China;Tujia Medicine Research Center in Hunan,Jishou 416000,Hunan China;Tujia Medicine Inheritance and Innovation Key Laboratory of the State Ethnic Affairs Commission,Jishou 416000,Hunan China)

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]湘西自然生物科技有限公司,湖南吉首416000 [3]吉首大学药学院,湖南吉首416000 [4]湖南省土家医药研究中心,湖南吉首416000 [5]土家医药传承与创新国家民委重点实验室,湖南吉首416000

出　　处：《吉首大学学报(自然科学版)》2024年第6期54-61,共8页Journal of Jishou University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(81960774);湖南省土家医药研究中心开放项目(201901);湖南省研究生科研创新项目(CX20240933)。

摘　　要：为了评价不同产地白花泡桐叶的质量,采用气相色谱-质谱联用技术对白花泡桐叶石油醚部位进行了定性分析,以角鲨烯为对照,测定了19批不同产地白花泡桐叶样品,并借助中药色谱指纹图谱相似度评价系统(2012 A版)作相似度评价,结合聚类分析和主成分分析评价白花泡桐叶的质量.结果表明,建立的白花泡桐叶GC-MS指纹图谱共标定了12个共有峰,分别为二氢猕猴桃内酯、植醇、乙酸十七酯、2-甲基二十八烷、角鲨烯、二十九烷、三十烷、三十六烷、α-生育酚、三十九烷、四十烷、β-谷甾醇;聚类分析将19批白花泡桐叶分为3大类;主成分分析得到2个主成分,累积方差贡献率为86.725%,S12号样品的主成分综合得分最高;主成分分析结果与聚类分析结果基本一致.To evaluate the quality of Paulownia fortunei leaves from different regions,gas chromatography-mass spectrometry was used to qualitatively analyze the petroleum ether fraction of Paulownia fortunei leaves.Squalene used as a control,the similarity evaluation system of traditional Chinese medicine chromatographic fingerprint(2012A version)used for similarity evaluation,cluster analysis and principal component analysis were combined to evaluate the quality.The results indicated that a total of 12 common peaks were identified in 19 batches of Paulownia fortunei leaves from various regions.These peaks corresponded to the following compounds:4,4,7a-Trimethyl-5,6,7,7a-tetrahydro-1-benzofuran-2(4H)-one,phytol,heptadecanoate,2-methyl octacosane,squalene,nonacosane,triacontane,hexatriacontane,α-Tocopherol,nonatriacontane,tetracontane,β-Sitosterol.Through cluster analysis,19 batches of Paulownia fortunei leaves were divided into 3 major categories.The cumulative variance contribution rate of the two principal components obtained from principal component analysis was 86.725%,and the results showed that sample S12 had a higher comprehensive score.The results of principal component analysis and cluster analysis were basically consistent.

关 键 词：白花泡桐叶 指纹图谱 气质联用 聚类分析 主成分分析 

分 类 号：R286.2[医药卫生—中药学]