IGFBP2通过AKT1/NF-κB通路调控胰腺癌细胞增殖和凋亡的机制  

IGFBP2 regulates proliferation and apoptosis of pancreatic cancer through AKT1/NF-κB pathway

作　　者：张虹[1] 叶丽君[2] 廖南生[3] 陈文晓[4] ZHANG Hong;YE Lijun;LIAO Nansheng;CHEN Wenxiao(Department of Laboratory Medicine,Taizhou First People's Hospital,Taizhou 318020,China;Department of Blood Transfusion,Taizhou First People's Hospital,Taizhou 318020,China;Department of General Surgery,Taizhou First People's Hospital,Taizhou 318020,China;Department of Gastroenterology,Taizhou First People's Hospital,Taizhou 318020,China)

机构地区：[1]台州市第一人民医院检验科,浙江台州318020 [2]台州市第一人民医院输血科,浙江台州318020 [3]台州市第一人民医院普外科,浙江台州318020 [4]台州市第一人民医院消化内科,浙江台州318020

出　　处：《健康研究》2025年第1期74-78,F0003,共6页Health Research

基　　金：浙江省台州市科学技术局项目(20ywa44)。

摘　　要：目的研究IGFBP2通过AKT1/NF-κB通路调控胰腺癌细胞增殖和凋亡的机制,为胰腺癌的诊断、靶向治疗和机制研究提供实验基础。方法收集64例胰腺癌患者和同期健康体检者100例,采用ELISA方法检测并比较2组血清中IGFBP2的表达水平;采用免疫组织化学方法检测胰腺癌患者癌组织和癌旁组织中IGFBP2的表达,RT-qPCR检测胰腺癌细胞系中IGFBP2的表达。CCK8法和流式细胞术评估细胞增殖、凋亡情况,Western blot法检测IGFBP2对AKT1/NF-κB信号通路的影响。结果胰腺癌患者的血清IGFBP2表达水平高于健康体检者,差异有统计学意义(P<0.05);IGFBP2诊断胰腺癌的曲线下面积为0.772(95%CI:0.696~0.849)。免疫组织化学染色方法显示,IGFBP2在胰腺癌患者癌组织明显高表达;RT-qPCR结果显示,IGFBP2在胰腺癌细胞中的表达水平高于人正常胰腺导管细胞系HPNE中的表达水平,且在AsPC-1细胞株中表达最高;差异均有统计学意义(P<0.01)。CCK8法和流式细胞术结果显示,抑制IGFBP2表达可以抑制细胞活力并促进细胞凋亡;Western blot检测结果显示,IGFBP2基因沉默后p-AKT、p-IkBa、p-P65蛋白表达水平均显著降低,差异均有统计学意义(P<0.05)。结论IGFBP2靶向调控AKT1/NF-κB通路,促进胰腺癌细胞增殖并抑制凋亡。Objective To study the mechanism of IGFBP2 regulating proliferation and apoptosis of pancreatic cancer through AKT1/NF-κB pathway,so as to provide an experimental basis for the diagnosis,targeted therapy and mechanism research of pancreatic cancer.Methods Serum samples were collected from 64 pancreatic cancer patients and 100 healthy controls.IGFBP2 expression levels in the serum were quantified using an ELISA assay and compared between the two groups.Immunohistochemical methods were used to analyze the expression of IGFBP2 in pancreatic cancer tissues and adjacent non-cancerous tissues,and RT-qPCR was used to detect the expression of IGFBP2 in pancreatic cancer cell lines.Cell proliferation and apoptosis were evaluated using the CCK8 assay and flow cytometry,respectively.Additionally,Western blot analysis was conducted to examine the effect of IGFBP2 on the AKT1/NF-κB signaling pathway.Results The level of serum IGFBP2 in pancreatic cancer patients was significantly higher than that in healthy people(P<0.05),and the area under the curve of IGFBP2 in diagnosis of pancreatic cancer was 0.772(95%CI:0.696~0.849).Immunohistochemical staining revealed that IGFBP2 was markedly overexpressed in pancreatic cancer tissues compared to adjacent tissues.RT-qPCR results showed that IGFBP2 expression in pancreatic cancer cells was significantly higher than in the normal human pancreatic ductal epithelial cell line HPNE,with the highest expression observed in the AsPC-1 cell line(P<0.01).Both the CCK8 assay and flow cytometry demonstrated that suppression of IGFBP2 expression inhibited cell viability and promoted apoptosis.The Western blot results showed that the expression levels of p-AKT,p-IkBa,and p-P65 proteins were significantly reduced after IGFBP2 gene silencing,and the differences were statistically significant(P<0.05).Conclusions IGFBP2 modulates pancreatic cancer cell proliferation and apoptosis by targeting the AKT1/NF-κB signaling pathway,suggesting its potential as a diagnostic marker and therapeutic target in p

关 键 词：胰腺癌 胰岛素样生长因子结合蛋白2 AKT1/NF-κB 增殖 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]