肠道微生物与乳房恶性肿瘤的因果关系:双样本孟德尔随机化分析  

Assessment of causal direction between gut microbiota andbreast malignancy:a two-sample mendelian randomization

作　　者：梁爽 连文静 梁鸿艺 李静蔚[2] LIANG Shuang;LIAN Wen-jing;LIANG Hong-yi;LI Jing-wei(The First Clinical Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Department of Breast and Thyroid Surgery,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学第一临床医学院,山东济南250014 [2]山东中医药大学附属医院乳腺甲状腺外科,山东济南250014

出　　处：《中国现代普通外科进展》2025年第2期108-113,共6页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金面上项目(NO.82374452)。

摘　　要：目的:探讨肠道微生物与乳房恶性肿瘤之间的因果关系,并通过孟德尔随机化(MR)分析确定特定因果微生物分类群。方法:对有关肠道微生物群和乳房恶性肿瘤的全基因组关联研究(GWAS)汇总统计数据,MiBioGen研究GWAS(N=18473)作为暴露样本,IEU Open GWAS数据库中乳房恶性肿瘤的GWAS(N=123579)作为结果样本。采用逆方差加权(IVW)、加权中位数和MR-Egger方法分析MR统计结果。结果:巴斯德菌目(Pasteurellales)丰度的升高会增加乳房恶性肿瘤患病风险(IVW PFDR<0.05,OR=1.21,95%CI:1.07~1.36),水平基因多效性和异质性检测验证不会对因果关系产生影响,留一法敏感性分析表明,已确定的因果关联不是由任何单个工具变量驱动的,因果关系为正向。结论:肠道中巴斯德菌目丰度升高可能会增加乳房恶性肿瘤的发生风险。Objective:Elucidating causal links between gut microbiota and breast malignancies using mendelian randomization(MR)to Identify specific causal microbial taxa.Methods:Genome-wide association studies(GWAS)summary statistics on gut microbiota and breast malignancies,MiBioGen studied GWAS(N=18473)as exposure samples,and GWAS(N=123579)of breast malignancies in the IEU Open GWAS database as outcome samples.The inverse variance weighted(IVW),weighted median and MR-Egger methods were used to analyze the MR statistical results.Results:Increased abundance of Pasteurellales increased the risk of breast malignancy(IVW PFDR<0.05,OR=1.21,95%CI:1.07~1.36),heterogeneity test and pleiotropy test showed no effect on causality,and the“leave-oneout”method showed that the identified causal association was not driven by any single instrumental variable.The causal relationship is positive.Conclusion:Elevated abundance of pasteurellales in the gut microbiota is associated with an increased risk of breast malignancies.

关 键 词：乳腺肿瘤 恶性 肠道菌群 孟德尔随机化 因果关系 

分 类 号：R737.9[医药卫生—肿瘤]