Immune effect and prognosis of transcatheter arterial chemoembolization and tyrosine kinase inhibitors therapy in patients with hepatocellular carcinoma  

作　　者：Yuan Guo Ru-Chun Li Wei-Li Xia Xiong Yang Wen-Bo Zhu Fang-Ting Li Hong-Tao Hu Hai-Liang Li 

机构地区：[1]Department of Minimal Invasive Intervention,The Affiliated Cancer Hospital of Zhengzhou University,Henan Cancer Hospital,Zhengzhou 450003,Henan Province,China [2]Department of Radiology,The Affiliated Cancer Hospital of Zhengzhou University,Henan Cancer Hospital,Zhengzhou 450003,Henan Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第7期3256-3269,共14页世界胃肠肿瘤学杂志(英文)

基　　金：Supported by Henan Province Natural Science Foundation,No.212300410403;Medical Education Research Project of Henan Province,No.Wjlx2021334。

摘　　要：The combination of transcatheter arterial chemoembolization(TACE)and tyrosine kinase inhibitors(TKIs)has shown broad prospects in prolonging the survival of patients with hepatocellular carcinoma(HCC).TACE and TKIs can affect the immune microenvironment in patients with HCC.AIM To determine the overall effects and differences between TACE and different TKIs combinations on the immune microenvironment.METHODS Data and immune cell profile test results from 213 HCC patients treated with TACE combined with apatinib,lenvatinib,sorafenib,or donafenib before and after 3 wk of treatment were collected.Monocytes were co-cultured with LM3 liver cancer cells,and their ability to inhibit cancer cell growth was analyzed using the MTT method and a nude mouse subcutaneous tumorigenesis experiment.Simulated combined therapy was done using an in situ liver cancer C57BL/6 male mouse model,and the immune response of tumor tissues was analyzed using immunohistochemistry.RESULTS Compared to before combination therapy,the proportion of programmed cell death protein 1(PD-1)+mononuclear cells and the number of CD4+T cells decreased in the TACE+apatinib group,while the number of absolute count of CD4+and CD8+T cells increased in the TACE+lenvatinib group.Furthermore,the number of regulatory cells decreased in the TACE+donafenib group,whereas the number of CD8+T and natural killer cells increased.Additionally,monocytes in the TACE combined with donafenib or lenvatinib groups had a stronger ability to inhibit cancer cell growth than those in the other groups.Combining TACE with donafenib or lenvatinib increased CD8+T cell infiltration into the tumor tissue.In addition,the proportion of PD-1+in CD8+cells,absolute CD8+T lymphocyte count,and regulatory T cells proportion were independent prognostic factors affecting the survival time of patients with HCC.CONCLUSION TACE,in combination with different TKIs,produces different immune responses.Specifically,TACE combined with donafenib or lenvatinib may induce strong anti-tumor immune responses.

关 键 词：Transcatheter arterial chemoembolization Hepatocellular carcinoma Tyrosine kinase inhibitors Immune microenvironment Immune responses 

分 类 号：R735.7[医药卫生—肿瘤]