壳寡糖对非酒精性脂肪性肝病的保护作用  

Protective effect of chitosan oligosaccharides on nonalcoholic fatty liver disease

作  者:赵康涛[1] 黄文琪 林颖珺 孙丽 赵坐都 黄宗锈[1] 吴珍红[1] 郑丽红[1] 林云[1] 韩尧跃 林秀芬 ZHAO Kang-tao;HUANG Wen-qi;Lin Ying-jun;SUN li;ZHAO Zuo-dou;HUANG Zong-xiu;WU Zhen-hong;ZHENG Li-hong;LIN Yun;HAN Yao-yue;LIN Xiu-fen(Fujian Center for Disease Control and Prevention(Fujian Academy of Preventive Medicine),Fuzhou 350001,China;Xiamen Humanity Hospital,Fujian 361009,China;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China;Xiamen Bluebay Science and Technology Co.,Ltd.,Fujian 361026,China)

机构地区:[1]福建省疾病预防控制中心,福州350012 [2]厦门弘爱医院 [3]上海交通大学药学院 [4]厦门蓝湾科技有限公司

出  处:《肝脏》2025年第1期91-94,共4页Chinese Hepatology

摘  要:目的探讨高含量、高脱乙酰度壳寡糖对非酒精性脂肪肝小鼠的辅助保护作用。方法将75只KM小鼠随机分为5组:3个样品试验组、模型组、阴性对照组。样品试验组和模型组每日摄入高脂饲料,阴性对照组予以常规饲料连续8周。第9周开始连续30 d经口灌胃样品试验组低(0.125 g)、中(0.25 g)、高(0.75 g)剂量的壳寡糖,阴性对照组和模型对照组给予等量纯水。在第31天处死动物,采血清测肝功能,观察肝组织外观性状并进行组织病理学检查。同时取部分肝组织匀浆,测定TG、GSH、MDA、TNF-α、TGF-β、IL-6等含量的变化。结果经口灌胃小鼠不同剂量的壳寡糖30 d后,中剂量组小鼠肝组织中的AST、TGF-β、TNF-α分别为(68.0±5.4)U/L、(62±5.0)pg/mL、(52±3.0)pg/mL,高剂量组肝组织中的TG(0.25±0.15)mmol/L、MDA(2.3±0.75)nmol/L、IL-6(2.8±0.3)pg/mL、ALT(35.5±2.7)U/L,模型组AST(89.5±9.4)U/L、TGF-β(75±4.2)pg/mL、TNF-α(60±5.3)pg/mL、TG(0.3±0.1)mmol/L(P<0.05);中、高剂量组GSH含量分别为(2.0±0.4)μmol/gprot和(2.1±0.8)μmol/gprot,较模型组的(1.5±0.9)μmol/gprot有所升高(P<0.05)。肝脏病理学检查结果表明:中、高剂量组小鼠体内肝脏中的脂滴分布范围小于模型组且面积积分低于模型对照组(P<0.05)。结论高含量、高脱乙酰度壳寡糖对非酒精性脂肪肝有辅助保护作用。Objective To study the protective effect of chitosan oligosaccharides on mouse nonalcoholic fatty liver disease.Methods 75 KM mice were randomly divided into 5 groups.Three sample groups and the model group were given with a high-fat diet daily for 8 weeks,while the negative group was given ordinary diet consecutively.Three sample groups of mice were given different doses of samples by oral gavage for 30 days after nine weeks.The animals of the negative control group and the model control group were simultaneously given equal amounts of pure water.At the end of the experiment,the animals were weighed and fasted for 16 hours.On the 31st day,the animals were anesthetized and killed.Blood samples were collected.We performed anatomical observation and histopathology examination on the liver tissue.Simultaneously take some liver tissue homogenate and examine changes in levels of TG,GSH,MDA,TNF-α、TGF-βand IL-6 in it.Results After administering different doses of chitosan oligosaccharides to mice by oral gavage for 30 days,the level of AST,TGF-βand TNF-α(68.0±5.4 U/L,62±5.0 pg/mL,52±3.0 pg/mL)in liver tissue of medium dose group,and the level of TG(0.25±0.15 mmol/L),MDA(2.3±0.75 nmol/L),IL-6(2.8±0.3 pg/mL),ALT(35.5±2.7 U/L)in the liver tissue of high-dose groups are decreased(AST 89.5±9.4 U/L,TGF-β75±4.2 pg/mL,TNF-α60±5.3 pg/mL,TG 0.3±0.1 mmol/L)(P<0.05)compared with the model group,while the concentration of GSH in the medium and high-dose groups(Medium^(2).0±0.4μmol/gprot,High-dose 2.1±0.8μmol/gprot)is increased(P<0.05),the results of liver pathology examination showed that the distribution,range,and area of lipid droplets in the medium and high-dose groups were lower than those in the model control group(P<0.05).Conclusion Chitosan oligosaccharides display an auxiliary protective effect on non-alcoholic fatty liver disease.

关 键 词:非酒精性脂肪性肝病 炎症 壳寡糖 免疫 肝功能 抗氧化 

分 类 号:R575.5[医药卫生—消化系统]

 

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