白皮杉醇对呕吐毒素致小鼠肝损伤的保护作用研究  

Protective Effect of Piceatannol on Liver Injury Induced by Deoxynivalenol in Mice

作　　者：程玉洁 刘国伟 陆恩庆 朱敏 CHENG Yujie;LIU Guowei;LU Enqing;ZHU Min(Institute of Animal Nutrition and Feed Science,Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,动物营养与饲料研究所,贵阳550025

出　　处：《中国畜牧兽医》2025年第3期1447-1454,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金青年科学基金项目(32202710)。

摘　　要：【目的】探究白皮杉醇(piceatannol,PIC)缓解呕吐毒素(deoxynivalenol,DON)诱导小鼠肝损伤的作用效果。【方法】选用18只5周龄初始体重为(19.3±0.8)g SPF级C57BL/6J雄性小鼠适应性饲养7 d,根据体重随机分为3组:对照组(CON)、呕吐毒素组(DON)以及白皮杉醇+呕吐毒素组(PIC+DON),每组6只,采用单笼饲养,试验期共14 d。CON组小鼠每天灌胃0.2 mL PBS溶液,连续14 d;DON组小鼠第1~7天连续灌胃0.2 mL PBS溶液,第8~14天连续灌胃0.2 mL呕吐毒素(3 mg/kg BW);PIC+DON组小鼠第1~7天连续灌胃0.2 mL白皮杉醇(40 mg/kg BW),第8~14 d连续灌胃0.2 mL呕吐毒素(3 mg/kg BW)。试验结束后采集小鼠肝脏组织及血液样本,测定肝脏中谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)、丙二醛(MDA)含量以及血清中总胆固醇(TC)、甘油三酯(TG)含量和谷草转氨酶(AST)、谷丙转氨酶(ALT)活性;通过HE染色观察肝脏组织形态学变化;利用实时荧光定量PCR检测肝脏中白细胞介素-6(IL-6)、IL-1β、B细胞淋巴瘤-2相关X蛋白(Bax)、半胱天冬蛋白酶-3(Caspase-3)、Caspase-9、Caspase-1、微管相关蛋白1轻链3β(LC3B)、核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、B细胞淋巴瘤-2(Bcl-2)和核因子E2相关因子2(Nrf2)mRNA相对表达量。【结果】与CON组相比,DON组小鼠体重显著降低(P<0.05),肝脏指数显著增加(P<0.05)。与DON组相比,PIC+DON组小鼠体重显著升高(P<0.05),肝脏指数显著降低(P<0.05)。小鼠肝脏组织病理切片结果显示,DON组小鼠肝索排列紊乱,肝细胞肿胀变性,内皮细胞脱落,炎性细胞增多,而PIC处理后病变程度明显改善。与CON组相比,DON组小鼠肝脏GSH-Px、CAT活性及T-AOC显著降低(P<0.05),肝脏MDA含量及血清TC、TG含量和AST、ALT活性均显著升高(P<0.05)。与DON组相比,PIC+DON组小鼠肝脏GSH-Px、CAT活性和T-AOC显著升高(P<0.05),肝脏MDA含量及血清TC、TG含量和AST、ALT活性均显著降低(P<【Objective】The purpose of this study was to investigate the effect of piceatannol(PIC)on liver injury induced by deoxynivalenol(DON)in mice.【Method】Eighteen 5-week-old SPF grade C57BL/6J male mice with an initial weight of(19.3±0.8)g after 7 d of adaptive feeding were randomly divided into 3 groups based on the body weight:Control(CON),DON and PIC+DON groups,with 6 mice in each group in a single cage.The experiment lasted for 14 d.Mice in CON group were given 0.2 mL PBS solution every day for 14 d.The mice in DON group were given 0.2 mL PBS solution continuously on days 1 to 7,and 0.2 mL DON(3 mg/kg BW)continuously on days 8 to 14.The mice in PIC+DON group were given 0.2 mL PIC(40 mg/kg BW)from days 1 to 7,and 0.2 mL DON(3 mg/kg BW)from days 8 to 14.Liver tissue and blood samples were collected after the experiment.The activities of glutathione peroxidase(GSH-Px)and catalase(CAT),total antioxidant capacity(T-AOC)and the content of malondialdehyde(MDA)in liver,the contents of total cholesterol(TC)and triglyceride(TG),and the activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum were determined.The changes of liver histomorphology were observed by HE staining.Real-time quantitative PCR was used to detect the relative mRNA expression of interleukin-6(IL-6),IL-1β,B-cell lymphomato-2-associated X protein(Bax),Caspase-3,Caspase-9,Caspase-1,microtubule associated protein 1 light chain 3β(LC3B),nuclear factor-κB(NF-κB),tumor necrosis factor-α(TNF-α),B-cell lymphoma-2(Bcl-2)and nuclear factor E2-associated factor 2(Nrf2)in liver.【Result】Compared with CON group,the body weight of mice in DON group was significantly decreased(P<0.05),and the liver index was significantly increased(P<0.05).Compared with DON group,the body weight of mice in PIC+DON group was significantly increased(P<0.05),and the liver index was significantly decreased(P<0.05).The pathological section results showed that the arrangement of liver cords was disordered,hepatocyte swelling and degeneration,endo

关 键 词：白皮杉醇 呕吐毒素 小鼠 肝损伤 

分 类 号：S853.74[农业科学—临床兽医学]