Ag/CuO/GO复合物的制备及其类酶活性和抗菌性能研究  

Synthesis of Ag/CuO/GO composites and their enzymaticactivity and antibacterial activity

作　　者：武华乙 傅晓涵 陈欢生 张润慈 吴宇寒 WU Huayi;FU Xiaohan;CHEN Huansheng;ZHANG Runci;WU Yuhan(Department of Pharmacy,Xiamen Medical College,Xiamen 361023,Fujian,China;Xiamen Key Laboratory of Traditional Chinese Medicine Bioengineering,Xiamen 361023,Fujian,China)

机构地区：[1]厦门医学院药学系,福建厦门361023 [2]厦门医学院厦门市中药工程重点实验室,福建厦门361023

出　　处：《工业催化》2025年第2期57-63,共7页Industrial Catalysis

基　　金：福建省中青年教师教育科研项目(JAT210493);厦门市医疗卫生指导性项目(3502Z200214ZD1324);国家大学生创新训练项目(202212631004)。

摘　　要：通过溶液法合成了Ag/CuO/GO纳米复合物,以3,3′,5,5′-四甲基联苯胺(TMB)为底物研究了复合物的过氧化物酶活性和动力学稳定性,采用平板计数法研究了复合物对大肠杆菌和金黄色葡萄球菌的抗菌活性。结果表明,Ag/CuO/GO纳米复合物具有很好的过氧化氢酶催化活性,其对底物TMB和H_(2)O_(2)的米氏常数均低于天然HPR过氧化物酶。在抗菌实验过程中,Ag/CuO/GO纳米复合物能催化H_(2)O_(2)产生具有优良杀菌效果的羟基自由基(·OH),从而显著提高纳米复合物的抗菌活性。In this paper,Ag/CuO/GO nanocomposites were synthesized by solution method.The peroxidase activity and kinetic stability of the composites were studied with 3,3′,5,5′-tetramethylbenzidine(TMB)as substrate.The antibacterial activity of the composites against Escherichia coli and Staphylococcus aureus was studied by plate counting method.The results showed that the Ag/CuO/GO nanocomposites had good peroxidase activity,and their Michaelis constant for substrates TMB and H_(2) O_(2) were lower than those of natural HPR peroxidase.In the process of antibacterial experiment,Ag/CuO/GO composites can catalyze H_(2) O_(2) to produce hydroxyl radicals(·OH)with excellent bactericidal effects,thus significantly improving the antibacterial activity of the nanocomposites.

关 键 词：酶学 纳米酶 羟基自由基 抗菌活性 Ag/CuO/GO 

分 类 号：TQ426.97[化学工程] Q55[生物学—生物化学]