MicroRNA-183通过结合LRIG1促进膀胱癌疾病进展的研究  

MicroRNA-183 Promotes Malignant Features of Bladder Cancer Cells Through Targeting LRIG1

作　　者：尤泓杰 王雍博 刘郅涵 沈栋杰 吕秀依[1] 张栋 严泽军[1] YOU Hongjie;WANG Yongbo;LIU Zhihan;SHEN Dongjie;LYU Xiuyi;ZHANG Dong;YANZejun(The First Affiliated Hospital of Ningbo University,Ningbo 315010,China;Graduate Training Base of Wenzhou Medical University(Ningbo),Ningbo315300,China)

机构地区：[1]宁波大学附属第一医院,浙江宁波315010 [2]温州医科大学研究生培养基础地(宁波),浙江宁波315300

出　　处：《肿瘤学杂志》2024年第12期1023-1030,共8页Journal of Chinese Oncology

基　　金：浙江省医药卫生科技计划项目(2021KY988)。

摘　　要：[目的]探讨微小RNA-183(microRNA-183,miR-183)对膀胱癌(bladder cancer,BCa)疾病进展的影响及作用机制。[方法]体外培养人BCa细胞株T24,分别/联合抑制细胞的miR-183和亮氨酸丰富重复免疫球蛋白样域蛋白1(leucine-rich repeats and immunoglobulin-like domains 1,LRIG1)的表达,将细胞分为对照(normal control,NC)、miR-183 inhibior、shLRIG1和miR-183 inhibitor+shLRIG1四组,qRTPCR检测miR-183和LRIG1 mRNA的表达,Western blot检测细胞中LRIG1和表皮生长因子受体(epidermal growth factor receptor,EGFR)的蛋白表达水平;CCK-8、划痕实验、Transwell法及流式细胞术分别检测细胞增殖、迁移、侵袭及凋亡情况。[结果]与NC组相比,miR-183敲减(miR-183 inhibior组)的T24细胞中LRIG1的表达增加而EGFR的表达降低(P<0.05),shLRIG1组中LRIG1表达降低而EGFR表达增加(P<0.05),shLRIG1+miR-183 inhibitor组逆转了单个因素敲减所导致的上述变化。此外,miR-183敲减后,T24细胞的增殖、迁移、侵袭水平降低而凋亡水平增加(P<0.05),shLRIG1组细胞的增殖、迁移、侵袭水平增加而凋亡水平降低(P<0.05),shLRIG1+miR-183 inhibitor组与NC组比较各项指标差异均无统计学意义。[结论]miR-183可能通过靶向结合LRIG1来调控膀胱癌细胞EGFR的表达,进而影响了膀胱癌细胞的生物学功能。[Objective]To investigate the effect and mechanism of microRNA-183(miR-183)on biological features of bladder cancer(BCa)cells.[Methods]The exprssions of miR-183 and leucine-rich repeats and immunoglobulin-like domains 1(LRIG1)were knocked down in human bladder cancer T24 cells.T24 cells were divided into 4 groups:NC group,miR-183 inhibior group,shLRIG1 group and miR-183 inhibitor+shLRIG1 group.The miR-183 and LRIG1 mRNA expressions were detected by qRT-PCR.The protein expressions of LRIGI and epidermal growth factor receptor(EGFR)were detected by Western blot.The cell proliferation,cell migration,cell invasion and apoptosis were detected by CCK-8,Scratch test,Transwell assay and flow cytometry,respectively.[Results]Compared with NC group,LRIG1 protein expression significantly increased and EGFR expression significantly decreased in the miR-183 inhibitor group(P<0.05),while LRIG1 protein expression significantly decreased and EGFR expression significantly increased in the shLRIG1 group(P<0.05);the shLRIG1+miR-183 inhibitor reversed the above changes.The cell proliferation,migration,invasion decreased,and the apoptosis increased in the miR-183 inhibitor group(P<0.05),while the cell proliferation,migration,and invasion increased,and the apoptosis decreased in the shLRIG1 group(P<0.05).There was no significant difference in the biological functions between the NC group and the shLRIG1+miR-183 inhibitor group.[Conclusion]MiR-183 may affect EGFR expression and regulate the bio-logical functions of bladder cancer cells through targeting LRIG1.

关 键 词：膀胱肿瘤 微小RNA-183 亮氨酸丰富重复免疫球蛋白样域蛋白1 增殖 迁移 侵袭 调亡 

分 类 号：R737.14[医药卫生—肿瘤] R73-1[医药卫生—临床医学]