产1,6-己二胺大肠杆菌的构建及其发酵强化  

Construction of 1,6-hexanediamine-producing Escherichia coli and its fermentation enhancement

作　　者：李文飞 刘伟 毛银 李国辉[1,2] 赵运英 邓禹 LI Wenfei;LIU Wei;MAO Yin;LI Guohui;ZHAO Yunying;DENG Yu(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China;Huaibei Mining Green Chemical New Materials Research Institute Co.Ltd.,Huaibei 235000,China)

机构地区：[1]江南大学,粮食发酵与食品生物制造国家工程研究中心,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122 [3]淮北矿业绿色化工新材料研究院有限公司,安徽淮北235000

出　　处：《食品与发酵工业》2025年第5期1-7,共7页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2021YFC2100700);国家自然科学基金项目(22008088,21877053);中国博士后科学基金资助项目(2020M681485,2021T140277);江苏省博士后科研资助计划项目(2020Z012)。

摘　　要：1,6-己二胺作为尼龙66的合成单体,其生产主要被国外垄断,传统的己二胺化学合成过程含有剧毒氰化物、技术壁垒高、生产工艺长,碳中和背景下如何利用生物法进行创新突破成为了难题,然而截止目前尚未发现天然的己二胺生物法合成途径。该研究以来源广泛的6-氨基己酸为底物,通过元件适配组装构建己二胺合成路径,并结合发酵强化以提高工程菌株己二胺产量。最终,通过筛选获得性能优良的羧酸还原酶MAB CAR L342E、单转氨酶HATA和双转氨酶组合PatA/VFTA,分别组合后工程菌株的初始己二胺产量可达到3.01 mg/L(DAH4)和8.50 mg/L(DAH37),在此基础上,开展发酵条件优化,1,6-己二胺产量分别达到64.33 mg/L和153.93 mg/L,较优化前分别提高了20.37倍和17.11倍。该研究成功构建了生物法己二胺合成路径,为助力国产化工产品突围提供了技术支持。The production of 1,6-hexanediamine as the synthetic monomer of nylon 66 is mainly monopolized by foreign countries,and the traditional chemical synthesis of hexanediamine contains highly toxic cyanide,high technical barriers,long production process,how to use the biological method to make innovative breakthroughs in the context of carbon neutrality has become a challenge.However,up to now,no natural hexanediamine synthesis pathway has been reported.In this study,6-aminohexanoic acid,a widely available substrate,was used to construct a synthetic pathway for hexanediamine through component-adapted assembly,and fermentation enhancement was combined to increase the yield of hexanediamine in engineered strains.Finally,excellent carboxylic acid reductase MAB CAR L342E,single transaminase HATA,and double transaminase combination PatA/VFTA were obtained through screening,and the initial hexanediamine yields of the engineered strains after combination could reach 3.01 mg/L(DAH4)and 8.50 mg/L(DAH37),respectively.On this basis,the fermentation conditions were optimized,and the yields of 1,6-hexanediamine reached 64.33 mg/L and 153.93 mg/L,which were 20.37 times and 17.11 times higher than those before optimization,respectively.This study has successfully constructed a pathway for the synthesis of hexanediamine by biological method,which provides technical support for the breakthrough of domestic chemical products.

关 键 词：1 6-己二胺 生物法合成 羧酸还原酶 转氨酶 发酵优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TQ226.31