生物基1,6-己二醇的全细胞催化及其过程强化  

Whole-cell catalysis of biobased 1,6-hexanediol and its fermentation enhancement

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作  者:李沂馨 毛银 刘伟[3] 李国辉 邓禹 LI Yixin;MAO Yin;LIU Wei;LI Guohui;DENG Yu(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China;Huaibei Mining Green Chemical New Materials Research Institute Co.Ltd.,Huaibei 235000,China)

机构地区:[1]江南大学,粮食发酵与食品生物制造国家工程研究中心,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122 [3]淮北矿业绿色化工新材料研究院有限公司,安徽淮北235000

出  处:《食品与发酵工业》2025年第5期8-14,共7页Food and Fermentation Industries

基  金:国家重点研发计划项目(2021YFC2100700);国家自然科学基金项目(22008088,21877053);中国博士后科学基金资助项目(2020M681485,2021T140277);江苏省博士后科研资助计划项目(2020Z012)。

摘  要:1,6-己二醇广泛应用于聚酯多元醇和UV固化材料等领域,然而传统化学合成主要被国外垄断,技术壁垒较高,碳中和背景下生物基己二醇的制备得到了广泛的关注。该研究旨在利用生物基己二酸为原料,通过元件适配组装在大肠杆菌中构建高效的己二醇合成路径,并结合发酵强化以提高生物基1,6-己二醇产量及其转化率。通过挖掘醛酮还原酶同工酶,并与来源于Mycolicibacterium smegmatis MC2155的羧酸还原酶进行适配组装,构建最优发酵工程菌株,相较原始菌株,1,6-己二醇的产量提高了6.4倍达到61.8 mg/L。在此基础上,开展发酵强化研究,确定了最优条件为TB培养基、底物质量浓度10 g/L、诱导时间2.5 h、初始糖质量浓度8 g/L,此时1,6-己二醇的产量达到818.16 mg/L,相较于初始菌株提高了84.7倍,转化率达到85%。该研究为1,6-己二醇的生物合成提供了新的思路。1,6-Hexanediol is widely used in the fields of polyester polyols and UV-curable materials,etc.However,the traditional chemical synthesis is mainly monopolised by foreign countries with high technological barriers,and the preparation of biobased hexanediols in the context of carbon neutrality has received wide attention.The aim of this study was to construct an efficient hexanediol synthesis pathway in Escherichia coli by component-adapted assembly using biobased adipic acid as the raw material,with fermentation enhancement to increase the yield of biobased 1,6-hexanediol and its conversion rate.The optimal fermentation strain was constructed by mining the aldo-keto reductase isozyme and assembled with the carboxylic acid reductase from Mycolicibacterium smegmatis MC2155,and the yield of 1,6-hexanediol was increased by 6.4-fold compared with that of the original strain to reach the titer 61.8 mg/L.On the basis of the fermentation enhancement study,the optimal conditions were determined to be the TB medium and the concentration of the substrate 10 g/L,induction time of 2.5 h,and initial sugar concentration of 8 g/L,at which the yield of 1,6-hexanediol reached 818.16 mg/L,which was 84.7-fold higher than that of the initial strain,and the conversion rate reached 85%.This study provides a new idea for the biosynthesis of 1,6-hexanediol.

关 键 词:1 6-己二醇 生物法合成 羧酸还原酶 醛酮还原酶 发酵优化 

分 类 号:TQ923[轻工技术与工程—发酵工程]

 

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