重组大肠杆菌透性化处理高效合成1,4-丁二胺  

Efficient synthesis of putrescine by permeabilization treatment of recombinant Escherichia coli

作　　者：丁博 刘伟 毛银 李国辉[1,2] 邓禹 DING Bo;LIU Wei;MAO Yin;LI Guohui;DENG Yu(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,JiangnanUniversity,Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China;Huaibei Mining Green Chemical New Materials Research Institute Co.Ltd.,Huaibei 235000,China)

机构地区：[1]江南大学粮食发酵与食品生物制造国家工程研究中心,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122 [3]淮北矿业绿色化工新材料研究院有限公司,安徽淮北235000

出　　处：《食品与发酵工业》2025年第5期15-21,共7页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2021YFC2100700);国家自然科学基金项目(22008088,21877053);中国博士后科学基金资助项目(2020M681485,2021T140277);江苏省博士后科研资助计划项目(2020Z012)。

摘　　要：1,4-丁二胺主要用于耐高温尼龙PA46的制备,然而其化学合成主要利用剧毒的氢氰酸和丙烯腈为原料,技术壁垒较高。针对丁二胺发酵法合成产量和产率较低的问题,该研究基于精氨酸到丁二胺的生物催化路线,通过透性化细胞处理提升物质的传输效率,并结合工程菌株培养和催化过程强化来提升丁二胺的合成能力。结果表明,乙醇透性化处理可显著解除传质限制,相比对照丁二胺产量提升50%以上达到14.92 g/L,在此基础上,确定了菌体的最优培养条件和最优催化工艺,在该条件下进行催化,丁二胺产量达到已有报道的最高水平88.67 g/L,摩尔转化率为0.81 mol/mol,生产效率为2.46 g/(L·h),为后续丁二胺的生物法开发利用奠定基础。Putrescine is mainly used in the preparation of high-temperature-resistant nylon PA46.Currently,it is mainly monopolized due to the high technical barriers derived from using highly toxic hydrocyanic acid and acrylonitrile as raw materials.To address the low yield and productivity in the biological synthesis of putrescine,this research improved the substance transfer efficiency via permeabilization of cells based on a biocatalytic route from arginine to putrescine,and the synthesis ability was enhanced through engineering strain cultivation and catalytic process enhancement.The results showed that the ethanol permeabilization treatment could significantly enhance the mass transfer limitation,and the putrescine yield was enhanced by more than 50%compared with the control,reaching 14.92 g/L.Meanwhile,the optimal cultivation conditions and the catalytic process were determined,and the putrescine yield reached the highest reported level of 88.67 g/L,with a molar conversion rate 0.81 mol/mol and a production efficiency of 2.46 g/(L·h),laying the foundation for the subsequent development of biobased putrescine.

关 键 词：丁二胺 全细胞催化 优化 生物合成 透性化细胞 

分 类 号：TQ226.31[化学工程—有机化工] O643.36[理学—物理化学] TQ920.6[理学—化学]