紫外诱变高产纤维素酶嗜盐菌株  

Ultraviolet mutagenesis of high yield cellulase producing halophilic strain

作　　者：王传旭[1,2] 于慧瑛 李云杰 王卓 杨静 杨瑾 何秋瑞 李新 WANG Chuanxu;YU Huiying;LI Yunjie;WANG Zhuo;YANG Jing;YANG Jin;HE Qiurui;LI Xin(Life Sciences Department,Yuncheng University,Yuncheng 044000,Shanxi,China;Shanxi Key Laboratory of Yuncheng Salt Lake Ecological Protection and Resource Utilization,Yuncheng University,Yuncheng 044000,Shanxi,China)

机构地区：[1]运城学院生命科学系,山西运城044000 [2]运城学院运城盐湖生态保护与资源利用厅市共建山西省重点实验室,山西运城044000

出　　处：《云南大学学报(自然科学版)》2025年第1期175-186,共12页Journal of Yunnan University(Natural Sciences Edition)

基　　金：山西省基础研究计划(自由探索类)(20210302123080);山西省科技创新人才团队项目(202204051001035);运城市科技计划项目基础研究(自由探索类-面上项目)(YCKJ-2023046)。

摘　　要：提高纤维素酶的活性和稳定性对解决能源和环境问题具有重要意义.从运城盐湖的湖水样本中筛选出具有纤维素酶活性的嗜盐菌株Y3.通过革兰氏染色、16S rRNA基因序列比对和系统发育分析初步鉴定其为Bacillus stercoris.研究发现Y3的最适培养条件为15.0%NaCl、45.0℃和pH=8.0,纤维素酶的最适反应条件为20.0%NaCl、45.0℃、pH=9.0,三氯甲烷对其活性影响较小.经紫外诱变后获得的Y3M菌株,其纤维素酶活性显著提高,在20.0%NaCl、50.0℃、pH=9.0条件下活性达到(102.4±3.7)U/mL,为诱变前的2.14倍.紫外诱变优化提高了Y3菌株纤维素酶的活性和稳定性,使其在复杂环境中具有更好的应用前景.Enhancing cellulase activity and stability is essential for addressing energy and environmental challenges.We isolated a halophilic strain,Y3,with cellulase activity from a water sample from Yuncheng Salt Lake.Preliminary identification was conducted through Gram staining,16S rRNA gene sequencing,and phylogenetic analysis,classifying Y3 as Bacillus stercoris.Optimal culture conditions for Y3 were determined as 15.0%NaCl,45.0℃,and pH 8.0,while the optimal reaction conditions for its cellulase were 20.0%NaCl,45.0℃,and pH 9.0,with minimal impact on activity from chloroform.After UV mutagenesis,the resulting strain Y3M showed significantly enhanced cellulase activity,reaching(102.4±3.7)U/mL under conditions of 20.0%NaCl,50.0℃,and pH 9.0,a 2.14-fold increase compared to the original strain.The UV-induced mutations improved both the activity and stability of Y3 cellulase,making the mutated strain and its cellulase more promising for applications in variable environments.

关 键 词：纤维素酶 运城盐湖 紫外诱变 稳定性 

分 类 号：Q93[生物学—微生物学]