鼻咽癌组织中miR-140-3p AGT5表达变化及临床意义  

Expression Changes and Clinical Significance of miR-140-3p AGT5 in Nasopharyngeal Carcinoma Tissue

作　　者：黄昌群 杨晓宇 胡玮 HUANG Changqun;YANG Xiaoyu;HU Wei(Xi'an Fifth Hospital,Shaanxi Xi'an 710082,China)

机构地区：[1]陕西省西安市第五医院耳鼻喉科,陕西西安710082 [2]陕西省西安高新医院耳鼻喉科,陕西西安710075 [3]陕西省西安大兴医院耳鼻咽喉颌面头颈外科,陕西西安710001

出　　处：《河北医学》2025年第2期297-301,共5页Hebei Medicine

基　　金：陕西省级科技计划项目,(编号:2020JM1079)。

摘　　要：目的:研究探讨鼻咽癌组织中微小RNA140-3p(miR-140-3p)、自噬相关基因5(AGT5)表达与病理参数及预后的关系。方法:选取102例鼻咽癌患者为研究对象,采用荧光定量PCR技术检测患者鼻咽癌组织和癌旁组织中的miR-140-3p、AGT5的表达情况,对比癌组织和癌旁组织中miR-140-3p、AGT5表达水平及相关性。并分析鼻咽癌组织中miR-140-3p、AGT5表达水平与患者临床病理特征参数的关系,随访3年,统计对比不同miR-140-3p、AGT5表达患者预后差异,Cox回归分析患者预后影响因素。结果:鼻咽癌组织中的miR-140-3p、AGT5表达水平低于癌旁组织(t=23.657、27.625,P=0.000、0.000)。鼻咽癌组织中miR-140-3p与AGT5表达呈显著正相关(r=0.614,P=0.000)。高分化、TNM分期为Ⅰ~Ⅱ期、无淋巴结转移患者中miR-140-3p、AGT5表达水平高于中低分化、TNM分期Ⅲ~Ⅳ期、有淋巴结转移患者(P<0.05)。患者3年总体生存率为75.49%(77/102)。miR-140-3p(≥0.97,n=49)、AGT5(≥1.46,n=48)高表达患者3年总体生存率为87.76%(43/49)、87.50%(42/48),高于miR-140-3p(<0.97,n=53)、AGT5(<1.46,n=54)低表达的64.15%(34/53)、64.81%(35/54),差异有统计学意义(Log-Rank 2=7.667、7.063,P=0.006、0.008)。分化程度低、TNM分期Ⅲ~Ⅳ期、淋巴结转移是鼻咽癌患者预后的独立危险因素,miR-140-3p、AGT5高表达是鼻咽癌患者预后的保护因素(P<0.05)。结论:miR-140-3p、AGT5在鼻咽癌患者中呈现低表达,与鼻咽癌的发生、发展及预后生存率有密切关联,可能共同参与鼻咽癌的进展。Objective:To investigate the correlation between the expression of microRNA140-3p(miR-140-3p)and autophagy related gene 5(AGT5)with pathological parameters and prognosis of nasopharyngeal carcinoma patients.Methods:A total of 102 nasopharyngeal carcinoma patients were selected as the research subjects.The expression of miR-140-3p and AGT5 in nasopharyngeal carcinoma tissues and adjacent tissues was detected by fluorescent quantitative polymerase chain reaction(qPCR)technology.The correlation of the expression levels of miR-140-3p and AGT5 in cancer tissues and adjacent tissues with pathological parameters and prognosis of nasopharyngeal carcinoma patients was assessed.After 3-year follow-up,the prognosis of patients with different expressions of miR-140-3 p and AGT5 was compared.Cox regression analysis was employed to analyze the factors affecting patient prognosis.Results:The expression levels of miR-140-3p and AGT5 in nasopharyngeal carcinoma tissues were significantly lower than those in adjacent tissues(t=23.657,27.625,P=0.000,0.000).There was a significant positive correlation between miR-140-3p and AGT5 expression in nasopharyngeal carcinoma tissues(r=0.614,P=0.000).The expression levels of miR-140-3p and AGT5 were significantly higher in patients with high differentiation,tumor-node-metastasis(TNM)stages Ⅰ~Ⅱ,and no lymph node metastasis than those with medium low differentiation,TNM stages Ⅲ~Ⅳ,and lymph node metastasis(P<0.05).The 3-year overall survival rate was 75.49%(77/102)among those patients.The 3-year overall survival rates of patients with high expression of miR-140-3p(≥0.97,n=49)and AGT5(≥1.46,n=48)were significantly higher than those with low expression of miR-140-3p(<0.97,n=53)and AGT5(<1.46,n=54)(87.76%[43/49]vs 64.15%[34/53],87.50%[42/48]vs 64.81%[35/54],Log-Rankc 2=7.667,7.063,P=0.006,0.008).Low differentiation,TNM stage Ⅱ-Ⅳ,and lymph node metastasis were independent risk factors for prognosis of patients with nasopharyngeal cancer.High expressions of miR-140-3p and AGT5 were

关 键 词：鼻咽癌 微小RNA140-3p 自噬相关基因5 病理特征 预后 

分 类 号：R739.63[医药卫生—肿瘤]