HJURP在人脑胶质瘤中的表达及意义:基于CGGA数据库的生信分析  

Expression and significance of HJURP in human glioma:a bioinformatics analysis based on the CGGA database

作　　者：孙玉晨[1] 杨生伟 王惠娟[3] SUN Yu-chen;YANG Sheng-wei;WANG Hui-juan(Department of Neurosurgery,The Second Hosipital of Hebei Medical University,Shijiazhuang 050000,China;Department of Neurosurgery,Zhangjiajie People's Hospital,Zhangjiajie 427000,China;Department of Neurology,The Second Hosipital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000 [2]张家界市人民医院神经外科,湖南427000 [3]河北医科大学第二医院神经内科,石家庄050000

出　　处：《中国临床神经外科杂志》2024年第12期724-728,共5页Chinese Journal of Clinical Neurosurgery

基　　金：河北省自然科学基金(H2022206078);河北省卫生健康委科研基金(20230047)。

摘　　要：目的探讨Holliday交叉识别蛋白(HJURP)在人脑胶质瘤中的表达及其与临床、病理特征及预后的关系。方法计算机检索中国脑胶质瘤基因组图谱(CGGA)数据库获取mRNAseq-325数据集中325例胶质瘤的转录组测序(RNA-seq)数据及临床资料,应用mRNAseq-693数据集中693例脑胶质瘤数据进行验证。利用R语言软件分析HJURP的表达和脑胶质瘤临床、病理特征的关系。使用Kaplan-Meier生存分析评估HJURP表达对人脑胶质瘤生存的影响。结果胶质瘤HJURP的表达水平明显上调(P<0.05);其表达水平与胶质瘤IDH基因型、1p19q共缺失状态、WHO病理分级有关(P<0.0001),与胶质瘤MGMT启动子甲基化无明显相关性(P>0.05)。Kaplan-Meier生存曲线分析显示,与HJURP高表达组相比,HJURP低表达组显示出显著的生存优势(P<0.0001)。GO、KEGG及GSVA分析显示HJURP参与脑胶质瘤细胞的有丝分裂调控、细胞周期、染色体分离等。结论HJURP在人脑胶质瘤中异常高表达,并与不良预后相关。这提示HJURP可作为人脑胶质瘤的生物标志物。Objective To explore the expression of Holliday Junction Recognition Protein(HJURP)in human glioma and its association with clinical,pathological characteristics,and prognosis of patients.Methods The mRNAseq-325 dataset containing RNAseq data and clinical information of 325 glioma cases was retrieved from the Chinese Glioma Genome Atlas(CGGA)database by computer.The dataset of 693 glioma cases in mRNAseq-693 was utilized for validation.The relationship between the expression of HJURP and the clinical and pathological characteristics of glioma was analyzed using R language software.Kaplan-Meier survival analysis was employed to assess the influence of HJURP expression on the survival of human glioma.Results The expression level of HJURP in glioma was significantly upregulated(P<0.05);its expression level was correlated with the IDH genotype of glioma,1p19q codeletion status,and WHO pathological grade(P<0.0001),but not significantly related to MGMT promoter methylation(P>0.05).Kaplan-Meier survival curve analysis demonstrated that the HJURP low-expression group exhibited a significant survival advantage compared to the HJURP high-expression group(P<0.0001).GO,KEGG,and GSVA analyses revealed that HJURP was involved in the regulation of mitosis,cell cycle,and chromosome segregation in glioma cells.Conclusion HJURP is aberrantly highly expressed in human glioma and is associated with an unfavorable prognosis.This indicates that HJURP can serve as a biomarker for human glioma.

关 键 词：脑胶质瘤 Holliday交叉识别蛋白(HJURP) 临床特征 病理特征 预后 

分 类 号：R739.41[医药卫生—肿瘤] Q786[医药卫生—临床医学]