血清VILIP-1和Pannexin1与急性脑梗死复发的关系及TLR4/NF-κB途径的参与机制  

Relationship between serum VILIP-1 and Pannexin1 and recurrence of acute cerebral infarction and the involved mechanism of TLR4/NF-κB pathway

作　　者：周哲 平立英 王康 吴力娟 ZHOU Zhe;PING Liying;WANG Kang;WU Lijuan(Department of Emergency,Xingtai Central Hospital,Xingtai,Hebei 054000,China;Department of Nephrology,Xingtai Central Hospital,Xingtai,Hebei 054000,China;Department of Neurology,Xingtai Central Hospital,Xingtai,Hebei 054000,China)

机构地区：[1]河北省邢台市中心医院急诊科,河北邢台054000 [2]河北省邢台市中心医院肾内科,河北邢台054000 [3]河北省邢台市中心医院神经内科,河北邢台054000

出　　处：《检验医学与临床》2025年第5期614-618,624,共6页Laboratory Medicine and Clinic

基　　金：河北省邢台市重点研发计划项目(2022ZC152)。

摘　　要：目的分析血清人视锥蛋白样蛋白-1(VILIP-1)和泛连接蛋白1(Pannexin1)与急性脑梗死复发的关系,并探讨Toll样受体4(TLR4)/核因子-κB(NF-κB)途径的参与机制。方法选取2020年1月至2021年1月该院收治的184例急性脑梗死患者作为观察组,根据神经功能损伤程度将观察组又分为轻度组(85例)、中度组(64例)和重度组(35例);根据1年后的随访结果又将观察组分为复发组和未复发组。另选取同期该院70例健康体检者作为对照组。检测所有研究对象血清VILIP-1、Pannexin1、TLR4、髓样分化因子88(MyD88)及NF-κB水平。采用点二列相关分析急性脑梗死患者血清VILIP-1、Pannexin1水平与急性脑梗死复发的相关性;采用Pearson相关分析急性脑梗死患者血清VILIP-1、Pannexin1水平与TLR4、MyD88、NF-κB水平的相关性。采用受试者工作特征(ROC)曲线分析血清VILIP-1和Pannexin1对急性脑梗死复发的预测价值。结果轻度组、中度组和重度组血清VILIP-1、Pannexin1水平比较,差异均有统计学意义(P<0.05),其中重度组血清VILIP-1、Pannexin1水平最高。点二列相关分析结果显示,血清VILIP-1和Pannexin1水平与急性脑梗死复发均呈正相关(P<0.05)。复发组血清VILIP-1、Pannexin1水平均明显高于未复发组,差异均有统计学意义(P<0.05);ROC曲线分析结果显示,血清VILIP-1和Pannexin1预测急性脑梗死复发的曲线下面积均>0.850,且VILIP-1和Pannexin1预测急性脑梗死复发的最佳截断值分别为8.09、5.08 mg/mL。轻度组、中度组和重度组血清TLR4、MyD88、NF-κB水平比较,差异均有统计学意义(P<0.05),其中重度组血清TLR4、MyD88、NF-κB水平最高。Pearson相关分析结果显示,急性脑梗死患者血清TLR4、MyD88、NF-κB水平与VILIP-1和Pannexin1水平均呈正相关(P<0.05)。结论血清VILIP-1、Pannexin1与急性脑梗死复发关系密切,并且对其复发具有较高的预测价值,且TLR4/NF-κB途径在急性脑梗死的发病和疾病Objective To analyze the relationship between serum VILIP-1 and Pannexin1 and the recurrence of acute cerebral infarction,and to explore the mechanism of Toll-like receptor 4(TLR4)/nuclear factor kappa-κB(NF-κB)pathway.Methods A total of 184 patients with acute cerebral infarction admitted to the hospital from January 2020 to January 2021 were selected as the observation group,according to the degree of nerve function injury,the observation group was further divided into mild group(85 cases),moderate group(64 cases)and severe group(35 cases).A total of 70 healthy physical examination subjects during the same period were selected as the control group.According to the follow-up results after 1 year,the observation group was divided into recurrence group and non-recurrence group.The serum VILIP-1,Pannexin1,TLR4,myeloid differentiation factor 88(MyD88)and NF-κB levels were detected in all subjects.Point biserial correlation analysis was used to analyze the correlation between serum VILIP-1,Pannexin1 levels and the recurrence of acute cerebral infarction patients.Pearson correlation analysis was used to analyze the correlation between serum VILIP-1,Pannexin1 levels and TLR4,MyD88,NF-κB levels in acute cerebral infarction patients.The receiver operating characteristic(ROC)curve was used to analyze the predictive value of serum VILIP-1 and Pannexin1 for the recurrence of acute cerebral infarction.Results There were significant differences in serum VILIP-1 and Pannexin1 levels among the mild group,moderate group and severe group(P<0.05),and the severe group had the highest serum VILIP-1 and Pannexin1 levels.Point biserial correlation analysis showed that the levels of serum VILIP-1 and Pannexin1 were positively correlated with the recurrence of acute cerebral infarction(P<0.05).The serum VILIP-1 and Pannexin1 levels in the recurrence group were significantly higher than those in the non-recurrence group(P<0.05).ROC curve analysis showed that the area under the curve of serum VILIP-1 and Pannexin1 for predicting the r

关 键 词：人视锥蛋白样蛋白-1 泛连接蛋白1 急性脑梗死 复发 Toll样受体4/核因子-κB途径 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R446.1[医药卫生—临床医学]