间歇禁食对大黄鱼生长、生理生化和肠道组织结构的影响  

Effects of intermittent fasting on growth, physiology and biochemistry,and intestinal histology of the large yellow croaker ( Larimichthys crocea )

作　　者：王晓萌 刘晃[2] 姜旭阳 张成林[2] 朱晨 龙丽娜[2] 徐金铖 马天利 WANG Xiaomeng;LIU Huang;JIANG Xuyang;ZHANG Chenglin;ZHU Chen;LONG Lina;XU Jincheng;MA Tianli(College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;Fishery Machinery and Instrument Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200092,China;College of Marine Science,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院渔业机械仪器研究所,上海200092 [3]福建农林大学海洋学院,福建福州350002

出　　处：《渔业现代化》2025年第1期64-72,共9页Fishery Modernization

基　　金：青岛海洋科技中心山东省专项经费(2022QNLM030001)。

摘　　要：为研究饥饿与恢复投喂对大黄鱼(Larimichthys crocea)的影响,试验以初始体质量为(122.62±11.08) g,体长为(17.9±1.04) cm的大黄鱼为样本,试验分成5组,分别为饥饿0 d(S0)、饥饿2 d(S2)、饥饿4 d(S4)、饥饿8 d(S8)、饥饿16 d(S16),然后再恢复投喂至第32天。结果显示:各组大黄鱼饥饿时体质量呈现降低趋势。恢复投喂后,在试验结束时所有饥饿组仅表现出部分补偿生长能力,其中S8组和S16组补偿生长能力较低。血清中甘油三酯以及碱性磷酸酶(AKP)、酸性磷酸酶(ACP)随饥饿时间的延长均降低。试验结束后,其指标在再投喂8 d后恢复不明显。此外,皮质醇浓度和超氧化物歧化酶(SOD)活性在饥饿8 d时升至最高,再投喂后恢复不显著。大黄鱼肠道组织结构与其生理生化变化趋势基本一致,肠绒毛高度和肌层组织厚度均在饥饿8 d时降至最低,而再投喂后恢复并不明显。肠道中IL-1β、IL-10和TNF-α基因表达量随饥饿升高,S4组最高,恢复投喂后表达量下降。研究认为:在该试验条件下,饥饿胁迫持续8 d及以上会对大黄鱼产生显著影响。因此为保证饲养效果,建议大黄鱼的饥饿时长宜控制在8 d以内。Whether in the wild or in captivity,large yellow croaker(Larimichthys crocea)may suffer from starvation.To study the effects of starvation and restoration feeding on large yellow croaker,The experiment was conducted with a sample of large yellow croaker with an initial body weight of(122.62±11.08)g and a body length of(17.90±1.04)cm.The fish were starved for 0,2,4,8 or 16 d(S0,S2,S4,S8,S16)and then feeding was resumed.The results are as follows:the body weights of the starved groups showed a decreasing trend,and the largest decrease was observed in group S8.After resumption of feeding,all the starved groups showed only partial compensatory growth capacity at the end of the experiment,among which the S8 and S16 groups showed lower compensatory growth capacity.Serum glucose,triglyceride,alkaline phosphatase(AKP)and acid phosphatase(ACP)tended to decrease with the extension of starvation time,and reached the lowest at 8 days of starvation.At the end of the experiment,its indicators did not recover significantly after 8d of refeeding.In addition,cortisol concentration and superoxide dismutase(SOD)activity rose to the highest at 8 days of starvation,while the recovery was not obvious after refeeding.The intestinal tissue structure of large yellow croaker was basically consistent with its physiological and biochemical trends,and the height of intestinal villi and the thickness of muscularis propria decreased to the lowest level at 8 days of starvation,and the recovery was not obvious after refeeding.IL-1β,IL-10 and TNF-αgene expression in the intestine increased with starvation,was highest in the S4 group and decreased after resumption of feeding.The results showed that:In summary,under the conditions of this experiment,starvation stress lasting for 8 days would have a significant effect on large yellow croaker.Therefore,in order to ensure the feeding effect,it is recommended that the length of starvation of the large yellow croaker should be controlled within 8 days.

关 键 词：大黄鱼 饥饿 生长性能 血清生化指标 肠道组织学 免疫指标 

分 类 号：S968.1[农业科学—水产养殖]