SP5在直肠腺癌组织中的表达及与患者临床病理特征和预后的关系  

Expression of SP5 in rectal adenocarcinoma tissues and its association with clinicopathological characteristics and prognosis

作　　者：曹蕾 王秀娟[2] 韩荣波 赵本新 CAO Lei;WANG Xiujuan;HAN Rongbo;ZHAO Benxin(Department of Clinical Laboratory,Wuhan Eighth Hospital,Wuhan 430013,China;Department of Critical Care Medicine,the Fourth Affiliated Hospital of Nanjing Medical University,Nanjing 210031,China;Department of Oncology,the Fourth Affiliated Hospital of Nanjing Medical University,Nanjing 210031,China;Department of Radiation Oncology,the Fourth Affiliated Hospital of Nanjing Medical University,Nanjing 210031,China)

机构地区：[1]武汉市第八医院检验科,430013 [2]南京医科大学第四附属医院重症医学科,210031 [3]南京医科大学第四附属医院肿瘤科,210031 [4]南京医科大学第四附属医院放疗科,210031

出　　处：《国际消化病杂志》2025年第1期20-24,共5页International Journal of Digestive Diseases

摘　　要：目的探究直肠腺癌组织中转录因子特异性蛋白5(SP5)的表达水平及与患者临床病理特征和预后的关系。方法选择2017年1月至2019年12月于南京医科大学第四附属医院和武汉市第八医院接受结直肠癌根治术的90例直肠腺癌患者作为研究对象,收集其经手术切除的直肠腺癌组织和癌旁组织(距离肿瘤组织边缘>2 cm)。采用实时荧光定量PCR法检测直肠腺癌组织和癌旁组织中SP5的表达水平,并分析SP5与直肠腺癌患者临床病理特征的关系。采用单因素和多因素Cox比例风险回归模型分析影响直肠腺癌患者预后生存的危险因素。结果直肠腺癌组织中SP5的相对表达量为1.10±0.16,显著高于癌旁组织(0.74±0.29),差异有统计学意义(P<0.05)。肿瘤分化程度为低分化、有神经侵犯、TNM分期为Ⅲ期的直肠腺癌患者组织中SP5的相对表达量均分别高于肿瘤分化程度为中/高分化、无神经侵犯、TNM分期为Ⅰ/Ⅱ期的患者(P均<0.05)。SP5高表达组的5年总生存率为37.21%,显著低于SP5低表达组(74.47%),差异有统计学意义(P<0.05)。单因素和多因素Cox回归分析结果显示,TNM分期、SP5均是影响直肠腺癌患者预后生存的独立危险因素(P均<0.05)。结论SP5在直肠腺癌组织中表达上调,且其高表达与患者预后不良密切相关。Objective This paper is to investigate the expression level of transcription factorspecific protein 5(SP5)in rectal adenocarcinoma tissues and its correlation with clinicopathological features and patient.Methods Ninety patients with rectal adenocarcinoma who underwent radical colorectal cancer surgery at the Fourth Affiliated Hospital of Nanjing Medical University and the Eighth Hospital of Wuhan from January 2017 to December 2019 were included.Rectal adenocarcinoma tissues and adjacent normal tissues(more than 2 cm from the tumor margin)were collected.Real-time quantitative PCR(qRT-PCR)was used to assess SP5 expression levels in both rectal adenocarcinoma tissues and adjacent tissues.The relationship between SP5 expression and clinicopathological features of patients was analyzed.Univariate and multivariate Cox proportional hazards regression models were employed to identify risk factors affecting overall survival.Results The relative expression level of SP5 in rectal adenocarcinoma tissues is 1.10±0.16,which is significantly higher than that in adjacent tissues(0.74±0.29),with a statistically significant difference(P<0.05).Patients with poorly differentiated tumors,neural invasion,or TNM stageⅢdisease show higher SP5 expression compared to those with moderately/well-differentiated tumors,no neural invasion,or TNM stageⅠ/Ⅱdisease(P<0.05).The 5-year overall survival rate for the high-SP5 expression group is 37.21%,which is significantly lower than that for the low-SP5 expression group(74.47%),with a statistically significant difference(P<0.05).Univariate and multivariate Cox regression analyses identifies TNM stage and SP5 expression as independent risk factors for prognosis(P<0.05).Conclusion SP5 is upregulated in rectal adenocarcinoma tissues,and its high expression is closely associated with poor patient prognosis.

关 键 词：直肠腺癌 特异性蛋白5 临床病理特征 预后 

分 类 号：R735.37[医药卫生—肿瘤]