尖果沙枣叶中3个新的没食子酸糖酯及其抗氧化活性与酪氨酸酶抑制活性  

Three new gallic acid sugaresters from Elaeagnus oxycarpa Schlechtend leaves and their antioxidant and tyrosinase inhibitory activities

作　　者：崔凤真 付建红 徐国燕[1,2,3] 阿依卡买尔·艾克拜尔 马畅达 CUI Feng-zhen;FU Jian-hong;XU Guo-yan;Ayekabayr·EKBAYR;MA Chang-da(Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,Urumqi 830017,China;Key Laboratory of Plant Stress Biology in Arid Land,Urumqi 830017,China;College of Life Sciences,Xinjiang Normal University,Urumqi 830017,China)

机构地区：[1]新疆特殊环境物种保护与调控生物学实验室,新疆乌鲁木齐830017 [2]干旱区植物逆境生物学实验室,新疆乌鲁木齐830017 [3]新疆师范大学生命科学学院,新疆乌鲁木齐830017

出　　处：《药学学报》2025年第2期434-441,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(32260226);新疆维吾尔自治区“天山创新团队计划”(2022D14004);新疆维吾尔自治区自然科学基金项目(2021D01A122)。

摘　　要：采用基于分离富集模式的反相多维液相制备色谱体系对尖果沙枣(Elaeagnus oxycarpa Schlechtend)叶水提取物进行分离纯化,得到5个化合物。通过NMR、MS、UV、IR等波谱数据结合文献确定化合物的结构,分别鉴定为尿嘧啶(1)、沙枣叶没食子酸糖酯B(2)、沙枣叶没食子酸糖酯A(3)、沙枣叶没食子酸糖酯C(4)、没食子酸(5),其中化合物2~4为新化合物,化合物1为首次从该植物中分离得到。运用二苯基苦基苯肼(DPPH)、2′-联氨双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、铁氰化钾还原法及酪氨酸酶催化左旋多巴氧化速率法评价化合物的体外抗氧化活性、酪氨酸酶抑制活性,发现化合物2~5具有抗氧化和抑制酪氨酸酶两种活性,其中化合物4抗氧化活性最强,其DPPH自由基清除能力(IC_(50))为3.59±0.06μmol·L^(-1),ABTS自由基清除能力(IC_(50))为10.04±0.20μmol·L^(-1),总还原能力均强于Vc;化合物3表现出较好的酪氨酸酶抑制活性,其IC_(50)为0.25±0.06 mmol·L^(-1)。Five compounds were isolated and purified from the water extract of Elaeagnus oxycarpa Schlechtend leaf by multi-dimensional reversed-phase preparative liquid chromatographic system based on the separation and enrichment model.Their structures were identified by spectral analysis such as NMR,MS,UV,IR and by comparison with literature information as 2,4(1H,3H)-pyrimidinedione(1),elaeagnussugarester B(2),elaeagnussugarester A(3),elaeagnussugarester C(4),gallic acid(5).Compounds 2−4 are new compounds,compound 1 was isolated from Elaeagnus oxycarpa Schlechtend for the first time.The antioxidant and antityrosinase activities of these compounds were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical method,the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)free radical method,the potassium ferricyanide reduction method and the colorimetric method with L-tyrosine as substrate.The results showed that compounds 2−5 have good antioxidant activities and inhibitory effect on tyrosinase.Compound 4 exhibited the most strong antioxidant activities,with IC_(50)=3.59±0.06μmol·L^(-1)for DPPH free radical scavenging ability,IC_(50)=10.04±0.20μmol·L^(-1)for ABTS free radical scavenging ability,and total reduction capacity of compound 4 was better than vitamin C respectively.Compound 3 possessed better inhibitory effect on tyrosinase with IC_(50)=0.25±0.06 mmol·L^(-1).

关 键 词：沙枣叶 没食子酸糖酯 结构鉴定 抗氧化活性 酪氨酸酶抑制活性 

分 类 号：R284[医药卫生—中药学]