2-羟丙基-β-环糊精降低BDE-47在紫贻贝中的蓄积并减缓其毒性作用  

2-Hydroxypropy-β-cyclodextrin reduced the accumulation and toxicity of BDE-47 in Mytilus galloprovincialis

作　　者：邹亮 刘红[1] 郭萌萌[2] 李风铃[2] 谭志军[2,3] 耿倩倩 ZOU Liang;LIU Hong;GUO Mengmeng;LI Fengling;TAN Zhijun;GENG Qianqian(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai,201306,China;Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,266071,China;State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,266071,China;Guangdong Provincial Key Laboratory of Quality&Safety Risk Assessment for Agro-products,Guangzhou,510640,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]农业农村部水产品质量安全检测与评价重点实验室,中国水产科学研究院黄海水产研究所,青岛266071 [3]海水养殖生物育种与可持续产出全国重点实验室,中国水产科学研究院黄海水产研究所,青岛266071 [4]广东省农产品质量安全风险评估重点实验室,广州510640

出　　处：《环境化学》2025年第2期666-676,共11页Environmental Chemistry

基　　金：中国水产科学研究院基本科研业务费(20603022024012,2020TD71);广东省农产品质量安全风险评估重点实验室开放课题(SZKF202303);山东省自然科学基金(ZR2020QD093)资助.

摘　　要：四溴联苯醚(BDE-47)因其高蓄积性和多毒性特点,引发严重的生态和健康风险,是国际上重点关注和管控的新污染物.研究通过比较BDE-47单独暴露和BDE-47+2-羟丙基-β-环糊精(HPCD)联合暴露处理的紫贻贝消化盲囊、性腺和鳃等组织中BDE-47蓄积和消除动态差异,测定氧化应激指标和组织损伤,结合分子对接模拟,探讨HPCD对BDE-47蓄积、分布和毒性效应的影响.结果表明,BDE-47在紫贻贝中的蓄积具有组织特异性;HPCD可显著降低BDE-47在紫贻贝各组织中的蓄积浓度,同时加快BDE-47的消除.HPCD降低了BDE-47在贻贝中的残留风险.GST、SOD、CAT活性和GSH、MDA含量变化分析表明,HPCD减轻了BDE-47对紫贻贝消化盲囊和性腺的氧化胁迫.BDE-47+HPCD处理组的综合生物标志物响应值(IBR)低于BDE-47处理组,组织病理损伤也较轻.分子对接显示HPCD减轻BDE-47对紫贻贝的毒性,可能是基于HPCD对BDE-47的屏蔽作用.研究结果为HPCD在减少海洋生物污染物积累和消除方面的应用提供数据支持.Tetrabromodiphenyl ether(BDE-47)is a typical emerging pollutants of international concern and control due to its high accumulative and multi-toxic characteristics,which poses serious ecological and health risks.To investigate the effects of 2-hydroxypropy-β-cyclodextrin(HPCD)on the accumulation,distribution and toxicity of BDE-47,the dynamic differences in the accumulation and elimination of BDE-47 in digestive gland,gonad,gill and other tissues of blue mussel Mytilus galloprovincialis exposed to BDE-47 and BDE-47+HPCD,as well as the oxidative stress indicators and tissue damages,were studied.Molecular docking simulation was used to simulate intermolecular interactions.Results showed that the accumulation of BDE-47 in M.galloprovincialis was tissue-specific;HPCD significantly reduced the accumulation,and accelerated the clearance of BDE-47 in mussel tissues.HPCD reduced the risk of BDE-47 residues in mussels.Changes in GST,SOD,and CAT activity,GSH and MDA content indicated that HPCD alleviated the oxidative stress of BDE-47 on digestive gland and gonad.The integrated biomarker response(IBR)index was lower in the BDE-47+HPCD treatment groups than that of BDE-47,and histopathological damage was also less severe.HPCD reduced the toxicity of BDE-47 to M.galloprovincialis,which may be due to the shielding effect of HPCD on BDE-47,based on molecular docking results.Results of this study could provide data to support the application of HPCD in reducing the accumulation and elimination of pollutants from marine organisms.

关 键 词：四溴联苯醚 (BDE-47) 羟丙基-Β-环糊精 (HPCD) 紫贻贝 蓄积 毒性 

分 类 号：X-1[环境科学与工程] O6[理学—化学]