野菊细胞色素P450基因家族全基因组鉴定及黄酮合酶基因分析  

Genome-Wide Identification of Cytochrome P450 Gene Family and Analysis of Flavonoid Synthase Genes in Chrysanthemum Indicum

作　　者：赵彬凯 陈诗 廖家豪 雷笛 张景景 刘义飞 ZHAO Binkai;CHEN Shi;LIAO Jiahao;LEI Di;ZHANG Jingjing;LIU Yifei(School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,武汉430065

出　　处：《世界科学技术-中医药现代化》2025年第1期24-35,共12页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会面上项目(32270231):菊属植物TS/CYP基因簇的起源进化机制及功能研究,负责人:刘义飞。

摘　　要：目的系统鉴定和分析野菊中细胞色素P450(CYP450)基因家族成员及其功能特性,为进一步研究其在类黄酮生物合成中的作用提供依据。方法基于二倍体野菊(Chrysanthemum indicum L.)基因组数据,利用生物信息学方法对野菊P450基因家族成员进行鉴定和分析。同时,结合转录组与类靶向代谢组筛选源于CYP93家族的黄酮合酶Ⅱ(FNSⅡ)基因,并利用实时荧光定量PCR(qPCR)检测其在不同组织中的表达水平。结果从野菊基因组中共鉴定到460个P450基因,归属于8个家族簇的43个家族。编码蛋白质的氨基酸数量为336-1538 aa,相对分子质量范围为38.01-175.00 kDa,等电点介于5.61-9.71。染色体定位分析显示,460个P450基因在9条染色体上呈不均匀分布,启动子区域中光响应类型元件数量最多。表达模式分析显示,两个FNSⅡ基因(CindChr2G00102820.1和CindChr8G00552890.1)在湖北黄石野菊叶组织中的表达量显著高于花。结论全面分析了野菊基因组中的P450基因家族,有助于进一步探究野菊P450基因家族在类黄酮生物合成中的功能。Objective Systematic identification and analysis of the cytochrome P450(CYP450)gene family members and their functions in Chrysanthemum indicum L.to provide a basis for further studies on their roles in the flavonoid synthesis pathway.Methods Based on the genomic data of a diploid Chrysanthemum indicum L.,the members of the P450 gene family were identified and analyzed using bioinformatics methods.Moreover,the transcriptome and quasitargeted metabolome analysis were combined to screen the flavone synthaseⅡ(FNSⅡ)genes from the CYP93 family,and their expression levels were tested in different tissues of C.indicum using real-time fluorescence quantitative PCR(qPCR).Results A total of 460 P450 genes were identified from the C.indicum genome,belonging to 43 families within 8 family clans.The encoded proteins ranged from 336 to 1538 amino acids in length,with a relative molecular mass between 38.01 to 175.00 kDa,and an isoelectric point between 5.61 to 9.71.Chromosome localization analysis indicated that these 460 P450 genes were unevenly distributed on nine chromosomes,with the highest number of light-responsive elements in the promoter regions.Expression pattern analysis showed that the expression levels of two FNSⅡgenes(CindChr2G00102820.1 and CindChr8G00552890.1)were significantly higher in leaf of C.indicum than in flower.Conclusion The comprehensive analysis of the P450 gene family in C.indicum is helpful to further elucidate the function of the P450 gene family in flavonoid biosynthesis.

关 键 词：野菊 P450基因家族 FNSⅡ 表达分析 

分 类 号：R-058[医药卫生]