胃癌术后伴焦虑患者肠道菌群特异性研究  

Specific study of intestinal flora in postoperative gastric cancer patients with anxiety state

作　　者：徐晓楠 朱爱松 王琦[4] 孙德森 盛静浩 于吉人[7] 金海龙[7] 秦逸杨 苏行 张光霁[1,2,3] XU Xiaonan;ZHU Aisong;WANG Qi;SUN Desen;SHENG Jinghao;YU Jiren;JIN Hailong;QIN Yiyang;SU Hang;ZHANG Guangji(School of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;Key Labortary of‘BloodStasis-Toxin’Syndrome of Zhejiang Province,Hangzhou 310053,China;Traditional Chinese Medicine‘Preventing Disease’Wisdom Health Project Research Center of Zhejiang Province,Hangzhou 310053,China;The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250002,China;School of Medicine,Ningbo University,Ningbo 315211,China;School of Medicine,Zhejiang University,Hangzhou 310011,China;The First Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou 310003,China)

机构地区：[1]浙江中医药大学基础医学院,杭州310053 [2]浙江省中医“瘀毒”证重点实验室,杭州310053 [3]中医“治未病”智慧健康浙江省工程研究中心,杭州310053 [4]山东中医药大学第二附属医院,济南250002 [5]宁波大学医学院,宁波315211 [6]浙江大学医学院,杭州310011 [7]浙江大学医学院附属第一医院,杭州310003

出　　处：《中华中医药杂志》2025年第1期354-358,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点研发计划中医药现代化研究重点专项(No.2019YFC1708700,No.2019YFC1708702);国家自然科学基金重点项目(No.82030119)。

摘　　要：目的:探究胃癌术后伴焦虑与不伴焦虑患者肠道菌群结构差异性,寻找胃癌术后伴焦虑患者肠道菌群特异性生物标志物。方法:83例胃癌三期D2根治术后患者焦虑组29例,非焦虑组54例,收集两组患者粪便标本,利用16S rDNA高通量测序技术研究分析两组之间肠道菌群差异性。结果:两组患者之间物种多样性与物种丰富度无明显差异;肠道菌群OUT、微生物群落构成和肠道菌群表型特征存在一定差异;在科水平上海洋杆菌科、巴克杆菌科物种丰度具有显著性差异(P<0.05);在属水平上Mar_丁二酰亚胺菌属、Pre_异普雷沃氏菌属、里克·阿利斯蒂普斯菌属菌群物种丰度具有显著性差异(P<0.05);焦虑组肠道优势菌有1种,是g_普雷沃菌科_UCG_001,非焦虑组肠道优势菌共有4种,分别是g_丁二酰亚胺菌目、f_马里尼菲拉克菌科、f_里克奈拉斯菌科、g_阿利斯提普菌目;两组在ko00760烟酸和烟酰胺代谢、抗霉素的生物合成、真核生物的核糖体生物发生、ko00430牛磺酸和次牛磺酸代谢、ko00513各类N-聚糖生物合成、MF0022半乳糖醛酸降解Ⅰ、MF0048丝氨酸降解、MF0005淀粉降解、MF0085尿素降解、MGB043醋酸酯合成Ⅰ、MGB041替代途径:futalosine途径多条代谢通路存在显著性差异;[瘤胃球菌]_转矩_组和UCG-003两个差异菌种与KO功能代谢呈正相关。结论:胃癌三期D2根治术后焦虑与非焦虑两组患者之间存在代表焦虑与非焦虑的特异菌种。Objective:To explore the structural differences of intestinal flora in patients with and without anxiety after gastric cancer surgery,and to search for specific biomarkers of intestinal flora in patients with anxiety after gastric cancer surgery.Methods:Fecal specimens were collected from 83 patients with gastric cancer in the anxiety group(29 cases) and the non-anxiety group(54 cases) after the third stage D2 radical operation.16S rDNA high-throughput sequencing technology was used to analyze the difference of intestinal flora between the two groups.Results:There was no significant difference in species diversity and species richness between the two groups.There were some differences in intestinal flora OUT,microbial community composition and phenotypic characteristics of intestinal flora.At the family level,the species abundance of Bac_Marinifilaceae and Bac_Rikenellaceae were significantly different(P<0.05).At the generic level,species abundance of Mar_Butyricimonas,Pre_Alloprevotella and Rik_Alistipes were significantly different(P<0.05).In the anxious group,there was one intestinal dominant bacteria,g_Prevotellaceae_UCG_001,while in the non-anxious group,there were four intestinal dominant bacteria,g_Butyricimonas,f_Marinifilaceae,f_Rikenellaceae and g_Alistipes.The two groups were studied in ko00760 Nicotinate and nicotinamide metabolism,ko01051 Biosynthesis of ansamycins,ko03008 Ribosome biogenesis in eukaryotes,ko00430 Taurine and hypotaurine metabolism,ko00513 Various types of N-glycan biosynthesis,MF0022 galacturonate degradation I,MF0048serine degradation,MF0005 starch degradation,MF0085 urea degradation,MGB043 Acetate synthesis I,MGB041 alternative pathway:futalosine pathway,There were significant differences in several metabolic pathways of futalosine pathway;There was a positive correlation between [Ruminococcus]_torques_group and UCG-003 with KO functional metabolism.Conclusion:There are specific strains representing anxiety and non-anxiety in patients with third stage D2 radical surgery for gastri

关 键 词：胃癌 焦虑 肠道菌群 高通量测序 特异菌 

分 类 号：R735.2[医药卫生—肿瘤]