胸苷磷酸化酶在宫颈癌进展中的作用初步探讨  

Preliminary exploration of the role of thymidine phosphorylase in cervical cancer

作　　者：陈珣[1] 郑真霞 阮雪茹[1] CHEN Xun;ZHENG Zhenxia;RUAN Xueru(Department of Obstetrics and Gynecology,Zhongshan Hospital Affiliated to Xiamen University,Xiamen 361004,China)

机构地区：[1]厦门大学附属中山医院妇产科,福建厦门361004

出　　处：《中国肿瘤外科杂志》2025年第1期80-86,共7页Chinese Journal of Surgical Oncology

摘　　要：目的分析胸苷磷酸化酶(TYMP)在宫颈癌进展中的作用,并初步探讨相关机制。方法质粒转染HeLa细胞,获得稳定过表达TYMP的细胞系和稳定敲减TYMP的细胞系。细胞增殖能力采用CCK8实验、EdU标记实验以及克隆形成实验进行检测;细胞迁移能力采用Transwell实验进行检测,并采用蛋白质组学分析稳定过表达TYMP的细胞与对照组细胞。结果宫颈癌HeLa细胞过表达TYMP后,细胞增殖能力明显增加(P<0.05),EdU阳性细胞数明显增加(P<0.05),迁移能力明显增加(P<0.001)。宫颈癌HeLa细胞敲减TYMP后,显著抑制细胞的增殖(P<0.001),EdU阳性细胞数显著减少(P<0.05),迁移能力显著受到抑制(P<0.001)。稳定过表达TYMP的细胞中脂代谢和血管生成通路上调,而ncRNA(非编码RNA)代谢及核糖体生物发生通路下调。结论TYMP在宫颈癌HeLa细胞中过表达对其增殖及迁移能力有促进作用,而敲除后其增殖及迁移能力显著受到抑制。其作用机制可能是通过调控脂代谢及血管生成而实现。Objective Thymidine phosphorylase is overexpressed in many cancers and also highly expressed in cervical cancer tissues.This article aims to analyze the role of TYMP on cervical cancer through in vitro experiments on cervical cancer HeLa cells and to preliminarily explore the possible mechanism.Methods By transfecting HeLa cells with plasmids,cells with stable overexpression of TYMP and cells with stale knockdown of TYMP were obtained.Cell proliferation ability was detected using CCK8 assay,EdU labeling assayand clone formation assay.Cell migration ability was detected using transwell assay.Cells with stable overexpression of TYMP and control cells were compared using proteomics analysis.Results The cell proliferation activity(P<0.05),the number of EdU positive cells(P<0.05),and the migration ability(P<0.001)were significantly increased in cervical cancer HeLa cells with overexpression of TYMP.After knocking down TYMP in cervical cancer HeLa cells,the cell proliferation was significantly inhibited(P<0.001),the number of EdU positive cells was significantly reduced(P<0.05),and the migration ability was significantly inhibited(P<0.001).In cells with stable overexpression of TYMP,lipid metabolism and angiogenesis pathways were upregulated,while ncRNA(non-coding RNA)metabolism and ribosome biogenesis pathways were downregulated.Conclusions Overexpression of TYMP in cervical cancer HeLa cells promotes the proliferation and migration ability,while knockdown significantly inhibits these abilities.The mechanism may be achieved by regulating lipid metabolism and angiogenesis.

关 键 词：宫颈癌 胸苷磷酸化酶 增殖 迁移 

分 类 号：R737.33[医药卫生—肿瘤]