Lin28基因在肝母细胞瘤中的表达及其对肝母细胞瘤细胞生物学功能的影响  

Expression of Lin28 gene in hepatoblastoma and its effect on biological functions of hepatoblastoma cells

作　　者：吕敏[1] 周婷婷[2] 刘嘉梅 张浩川[2] LYU Min;ZHOU Tingting;LIU Jiamei;ZHANG Haochuan(Department of Pediatric Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Department of Pediatric Surgery,the Second Affiliated Hospital&Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou 325027,China;The School of Laboratory Medicine and Life Science of Wenzhou Medical University,Wenzhou 325035,China)

机构地区：[1]温州医科大学附属第一医院小儿外科,浙江温州325015 [2]温州医科大学附属第二医院育英儿童医院小儿外科,浙江温州325027 [3]温州医科大学检验医学院(生命科学学院),浙江温州325035

出　　处：《温州医科大学学报》2025年第2期128-133,共6页Journal of Wenzhou Medical University

基　　金：温州市基础性科研项目(Y2020072)。

摘　　要：目的:研究Lin28基因在肝母细胞瘤(HB)中的表达情况,并探讨Lin28的表达对HB细胞生物学功能的影响。方法:综合分析癌症基因组图谱(TCGA)数据库和3个不同来源的基因表达数据库(GEO)数据集中Lin28基因的表达差异情况;通过转染构建的siRNA分别敲低HB细胞系Huh-6和HepG2的表达水平,通过细胞克隆集落形成实验、CCK-8、流式细胞术检测Lin28对HB细胞生物学表型的影响。结果:在TCGA数据库中,Lin28在大多数肿瘤中的表达均上调(P<0.05)。在GEO数据库中,Lin28在HB肿瘤组织较癌旁组织表达上调(P<0.05)。成功构建转染si-Lin28后,Lin28在mRNA及蛋白水平表达显著下降(P<0.05);敲低Lin28的表达可以显著抑制HB细胞系Huh-6和HepG2增殖能力(P<0.01),增加细胞凋亡率(P<0.05)。结论:Lin28在HB中表达上调,敲低Lin28的表达可以抑制细胞恶性表型,提示Lin28可能是HB一个潜在的治疗靶点。Objective:To investigate the expression of Lin28 gene in hepatoblastoma(HB)and explore its impact on biological phenotypes.Methods:The expression of Lin28 in pan-cancer and its expression in hepatoblastoma were analyzed using data from The Cancer Genome Atlas(TCGA)and three independent Gene Expression Omnibus(GEO)datasets.siRNA-mediated knockdown of Lin28 was performed in HB cell lines Huh-6 and HepG2.Small interfering RNAs(siRNAs)targeting Lin28 genes were constructed to transfect Huh-6 and HepG2 cells,and the interfering effect was verified at mRNA and protein levels.The effect of Lin28 on the biological phenotypes of HB cells was assessed by colony formation assays,CCK-8 assays,and flow cytometry.Results:In the TCGA database,Lin28 was found to be upregulated in the majority of tumors(P<0.05).In the GEO datasets,Lin28 expression was significantly higher in HB tumor tissues than adjacent non-tumor tissues(P<0.05).Successful transfection of si-Lin28 led to a significant reduction in Lin28 expression at both mRNA and protein levels(P<0.05).Knockdown of Lin28 markedly inhibited the proliferation of HB cell lines Huh-6 and HepG2(P<0.01)and increased the apoptosis rate(P<0.05).Conclusion:Lin28 is upregulated in HB,and its knockdown suppresses the malignant phenotype of HB cells,suggesting that Lin28 may serve as a potential therapeutic target for HB.

关 键 词：Lin28 肝母细胞瘤 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]