盐度胁迫对斑点叉尾鮰幼鱼生长及组织酶活性的影响  

Effects of Salt Stress on Growth and Tissue Enzyme Activity of Juvenile Channel Catfish(Ictalurus punctatus)

作　　者：段永强 刘洪岩[1,2] 王明华 钟立强[1,2] 苏超凡 刘炬[1] 陈校辉 张世勇 DUAN Yongqiang;LIU Hongyan;WANG Minghua;ZHONG Liqiang;SU Chaofan;LIU Ju;CHEN Xiaohui;ZHANG Shiyong(Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017;Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014)

机构地区：[1]江苏省淡水水产研究所,南京210017 [2]江苏省农业种质资源保护与利用平台,南京210014

出　　处：《中国农学通报》2025年第5期143-150,共8页Chinese Agricultural Science Bulletin

基　　金：财政部和农业农村部国家现代农业产业技术体系“国家特色淡水鱼产业技术体系”(CARS-46);江苏现代农业(大宗鱼类)产业技术体系“种质创新与苗种繁育创新团队”[JATS(2023)374]。

摘　　要：为探究盐度胁迫对斑点叉尾鮰鱼苗生长性能、组织抗氧化能力的影响,本试验采用等对数间距法设置6个盐度组(3.0、3.8、4.7、5.9、7.3、9.0)和1个对照组(盐度为0),每组3个重复。通过测量各组生长指标、4项理化指标及HE染色切片,评估其对不同盐度的耐受性。结果表明:当盐度为3.0、3.8、4.7时,鱼苗的生长性能及存活率无明显差异(P>0.05)。随着盐度的升高,鱼苗逐渐出现生长速度减缓、存活率降低现象,当盐度升至9.0时,存活率降至71%;肝脏组织中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)3种抗氧化酶活性随着盐度的升高表现为先升高后降低,且当盐度为4.7时,SOD、CAT活性显著增强(P<0.05),分别为1163.918、88.039 U/mg prot;鳃、肾脏组织中的3种抗氧化酶活性在各盐度组均无明显差异(P>0.05),各组织中的丙二醛(MDA)含量随盐度升高均有明显升高(P<0.05);苏木精和伊红染色(HE)切片结果显示,各组织在不同盐度条件下均有明显变化。以上结果表明不论低盐、高盐胁迫,均会对鱼体造成不同程度的损伤,且肝脏组织对盐度胁迫反应更为敏感。研究结果可为沿海滩涂地区科学培育斑点叉尾鮰鱼苗提供理论依据和基础数据。To investigate the effects of salinity stress on the growth and antioxidant capacity of channel catfish fry,we established six salinity groups(3.0,3.8,4.7,5.9,7.3,9.0)and one control group using the equidistant spacing method,the tolerance of each group to different salinity levels was assessed by measuring growth indexes,four physicochemical indexes,and H&E stained sections.The findings demonstrated that the growth performance and survival rates of fish fry at salinities of 3.0,3.8,and 4.7 did not differ significantly(P>0.05).The growth rate and survival rate of fish fry gradually slowed down with the increase of salinity.The survival rate fell to 71%when the salinity hit 9.0.As salinity increased,the activity of three antioxidant enzymes—superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-PX)—in liver tissue first increased and then decreased.SOD and CAT activities were considerably increased(P<0.05)at a salinity of 4.7,with values of 1163.918 U/mg prot and 88.039 U/mg prot,respectively.The Hematoxylin and Eosin(H&E)stained sections results revealed significant alterations in a variety of tissues under varying salinity settings.These findings suggest that both low and high salt stress can cause damage to fish bodies,with liver tissue being particularly sensitive to salt stress.This research provides a theoretical foundation and essential data for the scientific cultivation of channel catfish fry in coastal mudflats.

关 键 词：斑点叉尾鮰 生长性能 盐度胁迫 抗氧化酶 生理指标 HE切片 

分 类 号：S962[农业科学—水产养殖]