梯度醇沉雨生红球藻渣多糖的理化性质及体外抗氧化活性研究  

Physicochemical Properties and in Vitro Antioxidant Activity of Gradient Ethanol Precipitation Polysaccharides from Haematococcus pluvialis Residue

作　　者：肖金艳 宋瑞萍 王宝贝 林丽芹 谢友坪[1,5] 加晶 XIAO Jinyan;SONG Ruiping;WANG Baobei;LIN Liqin;XIE Youping;JIA Jing(School of Advanced Manufacturing,Fuzhou University,Quanzhou 362251,China;Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae,College of Oceanology and Food Science,Quanzhou Normal University,Quanzhou 362000,China;Key Laboratory of Inshore Resources and Biotechnology Fujian Province University,Quanzhou 362000,China;College of Chemical Engineering and Materials Science,Quanzhou Normal University,Quanzhou 362000,China;College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China;Beijing Key Laboratory of Microalgae Bioenergy and Bioresource,Beijing 100142,China;SDIC Microalgae Biotechnology Center,SDIC Biotechnology Investment Co.,Ltd.,State Development and Investment Corporation,Beijing 100034,China)

机构地区：[1]福州大学先进制造学院,福建泉州362251 [2]泉州师范学院海洋与食品学院,福建省海洋藻类活性物质制备与功能开发重点实验室,福建泉州362000 [3]近海资源生物技术福建省高校重点实验室,福建泉州362000 [4]泉州师范学院化工与材料学院,福建泉州362000 [5]福州大学生物科学与工程学院,福建福州350108 [6]微藻生物能源与资源北京市重点实验室,北京100142 [7]国家开发投资集团有限公司,国投生物科技投资有限公司,国投微藻生物科技中心,北京100034

出　　处：《食品工业科技》2025年第6期75-84,共10页Science and Technology of Food Industry

基　　金：福建省科技厅自然科学基金(2022J011101);泉州市科技项目(2020C027R);福建省海洋藻类活性物质制备与功能开发重点实验室(2022KF08)。

摘　　要：为探索雨生红球藻渣多糖的综合利用价值,本研究采用40%、60%和80%的乙醇梯度分离雨生红球藻渣多糖,依次得到HP40、HP60、HP80三个粗多糖组分,通过高效液相色谱(HPLC)、傅里叶红外光谱(FTIR)、凝胶色谱-示差-多角度激光光散射系统(HPSEC-MALS-RI)等方法对各组分多糖的单糖组成、分子量、结构、粒径和Zeta电位等理化特性进行分析,并测定其抗氧化活性。结果表明,乙醇浓度显著影响所沉淀雨生红球藻渣多糖的理化性质和抗氧化活性。醇沉粗多糖的得率和纯度随着乙醇浓度的增加而降低,并且乙醇浓度越高,沉淀的粗多糖分子量越低。本研究提取的粗多糖的纯度较高,其中HP40的总糖含量可达99.46%±0.015%。三种组分均包含Glc、Man、Gal、Ara、Rha、Xyl、Fuc、Glc-UA这8种单糖,但单糖组成及比例并不相同,与HP40相比,HP60和HP80还含有核糖,共有9种单糖。HP的粒径和Zeta电位分析结果表明,HP80的粒径最小(73.505±2.405 nm)且Zeta电位的绝对值最大(−11.6 mV)。抗氧化分析结果表明,HP80的抗氧化活性最强,且对羟自由基的清除率最高可达96%以上(10.0 mg/mL)。综上所述,梯度醇沉可以对雨生红球藻渣多糖进行初步分离,得到单糖组成、粒径、分子量、抗氧化活性各不相同的粗多糖。其中,40%乙醇所沉淀的粗多糖得率和糖含量最高,80%乙醇所沉淀的粗多糖分子量最小、抗氧化能力最强。To explore the comprehensive utilization value of polysaccharides from Haematococcus pluvialis residue,ethanol gradient precipitation(40%,60%and 80%)was used to isolate polysaccharides from H.pluvialis residue in this study.Three fractions with crude polysaccharides(HP40,HP60 and HP80)were obtained by 40%,60%and 80%ethanol precipitation successively.The monosaccharide composition,molecular weight,structure,particle size and Zeta potential of each fraction were analyzed by high performance liquid chromatography(HPLC),Fourier transform infrared spectroscopy(FT-IR),high pressure gel chromatography-multiple angle light scattering detector-differential refractive index detector(HPSEC-MALS-RI),and their antioxidant activity was also evaluated.Results showed that the physicochemical properties and antioxidant activity of polysaccharides precipitated from H.pluvialis residue were significantly affected by ethanol concentration.The yield and purity of the crude polysaccharides decreased with the increase of ethanol concentration.Furthermore,the lower molecular weight polysaccharides were precipitated by higher concentration of ethanol.High-purity crude polysaccharides were obtained in this study,in which,the total sugar content of HP40 was up to 99.46%±0.015%.All fractions contained 8 monosaccharides,including Glc,Man,Gal,Ara,Rha,Xyl,Fuc and Glc-UA,while the composition and proportion of monosaccharides were different.Compared with HP40,ribose was also involved in HP60 and HP80,which contained a total of 9 monosaccharides.HP80 possessed the smallest particle size(73.505±2.405 nm)and the largest Zeta potential absolute value(−11.6 mV).The results of antioxidant analysis showed that HP80 had the strongest antioxidant activity,and the scavenging rate of hydroxyl radicals was up to 96%(10.0 mg/mL).In summary,gradient ethanol precipitation method was effective to isolate polysaccharides from H.pluvialis residue preliminarily,and obtained polysaccharides with different monosaccharide composition,particle size,molecular weig

关 键 词：雨生红球藻渣 多糖 梯度醇沉 理化性质 抗氧化活性 单糖组成 分子量 粒径 

分 类 号：Q936[生物学—微生物学]