纳米钼对水稻硝态氮利用的影响  

Effect of nanomolybdenum on nitrate utilization in rice

作　　者：杨艳菊 浦佳玲 廖福兴 李莞意 王娟娟 钱晓晴[1,2] 张海鹏 YANG Yanju;PU Jialing;LIAO Fuxing;LI Wanyi;WANG Juanjuan;QIAN Xiaoqing;ZHANG Haipeng(College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225009,China;Key Laboratory of Arable Land Quality Monitoring and Evaluation,Ministry of Agriculture and Rural Affairs,Yangzhou University,Yangzhou 225009,China;Research Institute of Rice Industrial Engineering Technology/College of Agriculture,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225009 [2]扬州大学农业农村部耕地质量监测与评价重点实验室,江苏扬州225009 [3]扬州大学水稻产业工程技术研究院/农学院,江苏扬州225009

出　　处：《实验技术与管理》2025年第2期67-72,共6页Experimental Technology and Management

基　　金：国家自然科学基金项目(32472223,31901447);扬州大学农学院专业江苏省产教融合型品牌专业建设工程资助项目(2023-89);扬州大学“青蓝工程”骨干教师培养计划项目(2023-40);扬州大学大学生科创基金项目(XCX20230648)。

摘　　要：为了提高水稻对硝态氮的利用效率,通过水培实验研究了纳米钼对水稻氮代谢关键酶活性及硝态氮积累的影响。实验结果说明,与相同浓度的钼酸钠相比,纳米钼对水稻叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)活性的促进作用明显更大;钼酸钠和纳米钼能够促进水稻茎叶氮含量和水稻干物质积累,且纳米钼对水稻地上部和地下部干物质积累的促进效果显著优于钼酸钠。该项研究为提高水稻氮素吸收利用和促进水稻生长发育提供了一种解决方案,具有一定的应用前景。[Objective]This study primarily aimed to enhance rice’s utilization efficiency of nitrate nitrogen,which is a crucial nutrient for plant growth and development.By exploring the effects of molybdenum nanoparticles on key enzyme activities involved in nitrogen metabolism and nitrate nitrogen accumulation in rice,we aimed to provide a scientific basis for the development of new strategies to improve the nitrogen use efficiency of rice.This is particularly important for enhancing rice production and quality,as nitrogen is often a limiting factor in rice growth and yield.[Methods]To achieve this objective,a hydroponic experiment was conducted using rice plants.The experiment consisted of nine treatments to comprehensively evaluate the effects of molybdenum nanoparticles and sodium molybdate on rice nitrogen metabolism and growth.The treatments were as follows:1)no molybdenum application as the control group(CK);2)sodium molybdate at a concentration of 50μg Mo/L(T1);3)sodium molybdate at a concentration of 100μg Mo/L(T2);4)sodium molybdate at a concentration of 200μg Mo/L(T3);5)sodium molybdate at a concentration of 400μg Mo/L(T4);6)molybdenum nanoparticles at a concentration of 50μg Mo/L(T5);7)molybdenum nanoparticles at a concentration of 100μg Mo/L(T6);8)molybdenum nanoparticles at a concentration of 200μg Mo/L(T7);and 9)molybdenum nanoparticles at a concentration of 400μg Mo/L(T8).Rice plants were grown in a hydroponic system and subjected to these different treatments to investigate the effects of molybdenum nanoparticles and sodium molybdate on key enzyme activities involved in nitrogen metabolism,nitrate nitrogen accumulation,nitrogen content in rice stems and leaves,and rice dry matter accumulation.Specifically,we measured the activities of three key enzymes involved in nitrogen metabolism:nitrate reductase(NR),glutamine synthetase(GS),and glutamate synthase(GOGAT).We also assessed the nitrogen content in rice stems and leaves,as well as rice dry matter accumulation,to evaluate the overall effects of

关 键 词：水稻生长 氮含量 纳米钼 硝态氮利用 氮代谢 

分 类 号：X171.3[环境科学与工程—环境科学]