基于高分辨质谱技术的生物样本中蛋白同化雄性类固醇分析研究进展  

Research Progress on the Analysis of Anabolic Androgenic Steroids in Biological Samples Based on High Resolution Mass Spectrometry

作　　者：郭振硕 段文佳 刘玙 汤以绫 严慧 GUO Zhen-shuo;DUAN Wen-jia;LIU Yu;TANG Yi-ling;YAN Hui(Shanghai Key Laboratory of Forensic Medicine,Key Laboratory of Forensic Science,Ministry of Justice,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai 200063,China;College of Forensic Medicine,Kunming Medical University,Kunming 650500,China)

机构地区：[1]司法鉴定科学研究院、上海市法医学重点实验室、司法部司法鉴定重点实验室、上海市司法鉴定专业技术服务平台,上海200063 [2]昆明医科大学法医学院,云南昆明650500

出　　处：《法医学杂志》2024年第6期533-541,共9页Journal of Forensic Medicine

基　　金：中央公益课题资助项目(GY2022G-2);上海市法医学重点实验室资助项目(21DZ2270800);司法部司法鉴定重点实验室资助项目;上海市司法鉴定专业技术服务平台资助项目。

摘　　要：蛋白同化雄性类固醇(anabolic androgenic steroid,AAS)是一类拟雄性激素的人工合成的甾体激素,也是全世界滥用最为严重的一类兴奋剂。高分辨质谱技术凭借高分辨率、高灵敏度、高选择性及数据可追溯等优点在AAS检测中具有独特优势。高分辨质谱不仅可用于不同生物样本中AAS及其代谢物的定性定量分析,有效提升分析复杂样品的能力,增加分析结果的可靠性,还可通过结合体内外代谢模型,推断AAS代谢物,揭示代谢途径。本文对高分辨质谱技术在AAS分析方法和体内外代谢的研究进展进行综述,同时对其在司法鉴定领域的应用前景进行展望。Anabolic androgenic steroids(AASs)are a class of synthetic steroid hormones that mimic androgens,and they rank as the most widely abused doping agents worldwide.High resolution mass spectrometry(HRMS)has unique advantages in the detection of AASs due to its high resolution,high sensitivity,high selectivity and data traceability.HRMS can not only be used for the qualitative and quantitative analysis of AASs and their metabolites in different biological samples,effectively improving the ability to analyze complex samples and increasing the reliability of analytical results,but can also infer AASs metabolites and reveal metabolic pathways by combining in vitro and in vivo metabolic models.This paper reviews the research progress of HRMS in AASs analysis methods,in vitro and in vivo metabolism of AASs,and also explores its application prospects in the field of forensic science.

关 键 词：法医学 毒物分析 高分辨质谱 蛋白同化雄性类固醇 兴奋剂 生物样本 代谢物 综述 

分 类 号：R89[医药卫生—法医学] DF795.1[医药卫生—临床医学] D919.1[政治法律—诉讼法学]