L-抗坏血酸浸种对盐胁迫下甜菜种子萌发的影响  

Effects of L-ascorbic Selenite Immersion on Seed Germination of Sugar Beet Under Salt Stress

作　　者：田沁雨 胡华兵 王荣华 王茂芊[1] TIAN Qinyu;HU Huabing;WANG Ronghua;WANG Maoqian(College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080;Shihezi Agricultural Sciences Research Institute,Shihezi,Xinjiang 832000)

机构地区：[1]黑龙江大学现代农业与生态环境学院,哈尔滨150080 [2]石河子农业科学研究院,新疆石河子832000

出　　处：《中国农学通报》2025年第6期53-59,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金面上项目“转录因子BvERF61调控甜菜耐盐分子机制研究”(32272148);国家糖料产业体系子项目“甜菜优良单胚不育系选育与优势组合创新”(CARS-170113-1);国家糖料产业体系子项目“甜菜抗根腐病及抗丛根病品种筛选与鉴定”(CARS-17011004);国家糖料产业体系项目“甜菜种质资源鉴定与新种质创制”(CARS-170102);黑龙江省博士后面上项目“不同根冠比甜菜转录组-蛋白组联合分析”(LBHZ22246)。

摘　　要：为研究在盐胁迫条件下,不同浓度和浸泡时长的L-抗坏血酸处理对甜菜种子发芽性能的影响,于2023年9—12月,以甜菜品种‘TD802’为供试材料,在黑龙江大学现代农业与生态环境学院甜菜遗传育种重点实验室开展了试验,使用100 mmol/L的NaCl溶液模拟盐胁迫环境,选取3个浓度的L-抗坏血酸溶液,在3个不同的浸泡时长下对甜菜种子进行处理,通过测量处理后的种子发芽势率等指标来评估各处理的效果。结果显示,对于盐胁迫处理后的‘TD802’甜菜种子,浸泡处理8 h后的种子活力指标普遍优于浸泡16 h和24 h的处理。其中,使用浓度为300 mg/L的L-抗坏血酸溶液浸泡处理8 h的效果最为显著,其发芽势率、发芽指数、活力指数较对照组(CK)分别提高了74.33%、20.33%、48.6%和102.97%,同时电导率降低了113.56%。研究发现,L-抗坏血酸的浓度与浸泡时间的组合对种子的引发效果存在显著差异,且浸泡时间对于缓解盐胁迫下的种子发芽状况表现出了较高的敏感性。The purpose of this experiment is to investigate the effects of different concentrations of L-ascorbic acid treatment on the germination of'TD802'sugar beet single-seed under salt stress conditions,with the aim of screening for suitable priming agents for sugar beet seeds.In the experiment,a 100 mmol/L NaCl solution was used to simulate a salt stress environment,and three concentrations of L-ascorbic acid solution were selected to treat the sugar beet seeds at three different soaking durations.The effectiveness of each treatment was evaluated by directly measuring indicators such as the germination potential rate of the treated seeds.The results indicated that for the'TD802'sugar beet seeds treated under salt stress,the seed viability indicators after 8 hours of soaking treatment were generally better than those after 16 hours and 24 hours of soaking.Among them,the effect of soaking with a 300 mg/L L-ascorbic acid solution for 8 hours was the most significant.Compared with the control group(CK),its germination potential rate,germination index,and vigor index increased by 74.33%,20.33%,48.6%,and 102.97%respectively,while the electrical conductivity decreased by 113.56%.

关 键 词：甜菜 种子引发 盐胁迫 L-抗坏血酸 发芽势率 

分 类 号：S566.3[农业科学—作物学]