甘露醇促进人肺鳞癌细胞系NCI-H292凋亡  

Mannitol promotes the apoptosis of human lung squamous carcinoma cell line NCI-H292

作　　者：李黄羿[1] 黄道航 张文玉 黄鼎铭[1] 黄其春[1] LI Huangyi;HUANG Daohang;ZHANG Wenyu;HUANG Dingming;HUANG Qichun(Department of Pharmacy,Guangxi Medical University Affiliated Cancer Hospital,Nanning 530021,China)

机构地区：[1]广西医科大学附属肿瘤医院药剂科,广西南宁530021

出　　处：《基础医学与临床》2025年第3期336-340,共5页Basic and Clinical Medicine

基　　金：2022年度广西高校中青年教师科研基础能力提升项目(2022KY0106);2022年度广西壮族自治区卫生健康委西医类自筹经费科研课题(Z-A20220676)。

摘　　要：目的探讨20%甘露醇注射液对人肺鳞癌细胞系NCI-H292增殖和凋亡的作用及其可能机制。方法使用20%及以下不同浓度甘露醇处理NCI-H292细胞,CCK-8法检测NCI-H292细胞增殖和活性;流式细胞测量术检测细胞凋亡;Western blot检测线粒体凋亡途径相关蛋白Bcl-2和Bax的表达。结果与对照组比较,甘露醇呈剂量和时间依赖性抑制细胞增殖(P<0.05);20%甘露醇可诱导细胞凋亡(P<0.05);经20%甘露醇处理后的细胞中Bcl-2的表达水平均显著降低,Bax的表达水平均明显升高(P<0.05)。结论临床上常用的20%甘露醇注射液可以诱导肺鳞癌细胞系NCI-H292凋亡,其机制与线粒体途径相关蛋白Bcl-2、Bax有关。Objective To investigate the effects of 20% mannitol injection on the proliferation and apoptosis of human lung squamous carcinoma cell line NCI-H292 and its possible mechanisms.Methods NCI-H292 cells were incubated with 20% and lower concentrations of mannitol.Cell proliferation and viability were detected by CCK-8 assay.Cell apoptosis was detected by flow cytometry and the expression of mitochondrial apoptosis pathway-related proteins Bcl-2 and Bax was detected by Western blot.Results Compared with the control group,mannitol inhibited cell proliferation in a dose-and time-dependent manner(P<0.05),20% mannitol could induce cell apoptosis(P<0.05).The expression level of Bcl-2 was significantly reduced,while that expression level of Bax was significantly increased in cells incubated with 20%mannitol(P<0.05).Conclusions Clinically used 20% mannitol injection can induce apoptosis of lung squamous carcinoma cell line NCI-H292.Its mechanism is related to mitochondrial pathway-related proteins Bcl-2 and Bax.

关 键 词：甘露醇 肺鳞癌 凋亡 BCL-2 BAX 

分 类 号：R73-36[医药卫生—肿瘤] R982[医药卫生—临床医学]