天花粉蛋白对胃癌细胞生物活性及Livin mRNA的影响  

Effects of Trichosanthin on the biological activity of gastric cancer cells and the level of Livin gene mRNA

作　　者：吴殿超[1] 霍志斌[1] 赵笑枫 李巍伟 WU Dian-chao;HUO Zhi-bin;ZHAO Xiao-feng;LI Wei-wei(Department of Gastrointestinal Cancer,Xingtai People's Hospital,Hebei Province,Xingtai 054031;Department of Oncology,Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China)

机构地区：[1]河北省邢台市人民医院胃肠肿瘤外科,河北邢台054031 [2]华北理工大学附属医院肿瘤科,河北唐山063000

出　　处：《解剖科学进展》2024年第6期564-567,共4页Progress of Anatomical Sciences

基　　金：河北省医学科学研究项目(20190104)。

摘　　要：目的探讨天花粉蛋白(TCS)对胃癌细胞生物活性及Livin基因mRNA水平的影响。方法将人类低分化胃癌细胞株BGC-823分为对照组和干预组,对照组无药物处理,干预组经过7、14和28μmol/L浓度的TCS处理,显微镜观察BGC-823细胞形态,MTT观察活力,流式细胞仪观察凋亡率,免疫印迹和qRT-PCR检测Livin蛋白和mRNA水平。结果7、14和28μmol/L天花粉蛋白使BGC-823细胞株形态逐渐变形皱缩,透明度降低,生长能力减慢,体积变大,细胞碎片漂浮,细胞活力随着TCS剂量升高而降低,呈现时间依赖性,BGC-823细胞凋亡率均高于对照组(P<0.05),Livin蛋白和mRNA水平低于对照组。结论天花粉蛋白抑制胃癌细胞BGC-823增殖,加快凋亡,与抑制Livin基因水平相关。Objective To explore the effect of Trichosanthin(TCS)on the biological activity of gastric cancer cells and the level of Livin gene.Methods Human poorly differentiated gastric cancer cell line BGC-823 was divided into control group and intervention group.The morphology of BGC-823 cells was observed under microscope,cell vitality was studied by MTT,apoptosis by flow cytometry,immunoblotting and qRT-PCR were used to determine Livin protein and mRNA.Results 7,14 and 28μmol/L TCS resulted in BGC-823 cells shape gradually deformed and shrunk,reduced transparency,slowed growth ability,decreased cell viability in dose dependent manner,the increased apoptosis rate of BGC-823 cells,the lower expression levels of Livin protein and mRNA in BGC-823 cells(P<0.05).Conclusion TCS inhibits the proliferation of human poorly differentiated gastric cancer BGC-823 cells and accelerates the cell apoptosis,which is related to the inhibition of Livin gene.

关 键 词：天花粉蛋白 BGC-823 胃癌 生物活性 LIVIN基因 

分 类 号：R735.2[医药卫生—肿瘤]