不同片烟醇化过程中物质变化的共性规律分析  

General rules for leaf chemical changes in different tobacco strips during aging

作　　者：郑庆霞[1] 周瑢[2] 陈森林[2] 李泽锋[1] 陶红[2] 刘萍萍[1] 林云[2] 李月灵 周会娜[1] ZHENG Qingxia;ZHOU Rong;CHEN Senlin;LI Zefeng;TAO Hong;LIU Pingping;LIN Yun;LI Yueling;ZHOU Huina(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;China Tobacco Guangdong Industrial Co.,Ltd.,Guangzhou 510610,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]广东中烟工业有限责任公司,广州市510610

出　　处：《烟草科技》2025年第2期41-51,共11页Tobacco Science & Technology

基　　金：广东中烟工业有限责任公司科研项目“代谢组学技术在不同香型烟叶质量评价上的应用研究”(粤烟工[2020]科字第19号)。

摘　　要：为了从代谢组学角度系统分析片烟醇化过程中小分子物质成分的变化规律,以广东中烟工业有限责任公司烟叶醇化库存放的不同香型产区的单打或混打片烟为研究对象,采用基于超高效液相色谱-四级杆飞行时间质谱的非靶向代谢组技术,对跨度2年7月(2018年8月至2021年4月)、涉及8个醇化时间点的烟叶样品进行分析。结果表明:不同片烟的代谢组表现出较为一致的随醇化时间进行变化的规律;以醇化时间为性状的WGCNA分析将全部代谢物分为27个模块,其中与醇化早期和末期相关的模块较多;从5个与4个以上醇化时间点均有显著关联性的模块中筛选到核心代谢物59种,涉及脂类、香气成分、二肽、萜、黄酮等,其中脂质含量随烟叶醇化过程逐渐降低,一些萜类及小分子的酸、酮等香气成分随醇化过程逐渐升高;随着醇化的进程,烟叶表面微生物活跃,二肽类成分表现出逐渐升高的趋势。本研究为进一步了解烟叶醇化过程中的化学变化提供了重要数据,同时核心代谢物的鉴定对于监控醇化过程、控制醇化质量和优化醇化时间等具有一定的指导意义。To achieve target metabolome changes in flue-cured tobacco strips during aging,tobacco strips from different domestic production areas were sampled and studied using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry at 8 sampling time points across 31 months(August 2018 to April 2021)to track their natural aging process in the aging warehouse of China Tobacco Guangdong Industrial Limited Corporation.The results showed that the metabolome of different tobacco strips showed a consistent pattern of chemical changes with aging.The weighted gene co-expression network analysis(WGCNA)divided all the metabolites into 27 modules with the characteristics of the aging duration,and more modules were significantly correlated with the early or late stages of aging.From the 5 modules found to be significantly correlated with at least 4 of the aging durations,59 hub metabolites were identified,including lipids,aroma components,dipeptides,terpenes,and flavonoids.More specifically,the lipid contents gradually decreased,the contents of some terpenes and small molecule acids,ketones and other aroma components gradually increased,the microorganisms on the surface of tobacco leaves became more active,and the contents of dipeptides gradually increased with the progress of aging.This study provides important data for a comprehensive understanding of the chemical changes in tobacco leaves during natural warehouse aging,and the hub metabolites identified are of great application for monitoring the aging process,controlling the quality of aged tobacco,and optimizing the aging duration.

关 键 词：片烟醇化 香型风格 代谢组学 加权基因共表达网络分析 

分 类 号：TS413[农业科学—烟草工业]