畜禽屠宰环节中单增李斯特菌的分离鉴定及分离株生物学特性分析  

Identification and characteristics analysis of Listeria monocytogenes in slaughter process livestock and poultry

作　　者：范子秋 曹青 肖敏 王子坚[2] 马永辉 芝吉 赵学慧 张浩浩 何曾文 邓静 马金锐 崇倩 张坤中 薛慧文[1] 苟惠天[1] FAN Ziqiu;CAO Qing;XIAO Min;WANG Zijian;MA Yonghui;ZHI Ji;ZHAO Xuehui;ZHANG Haohao;HE Zengwen;DENG Jing;MA Jingrui;CHONG Qian;ZHANG Kunzhong;XUE Huiwen;GOU Huitian(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Animal Disease Control and Prevention Center of Gansu Province,Lanzhou 730030,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]甘肃省动物疫病预防控制中心,甘肃兰州730030

出　　处：《畜牧与兽医》2025年第3期58-65,共8页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31960726,32060822);甘肃省现代丝路寒旱农业科技支撑项目(GSLK-2022-17);甘肃省农业科技支撑项目(KJZC-2024-20);甘肃省青年博士支持项目(2024QB-077)。

摘　　要：通过对畜禽屠宰环境和畜禽肉中单增李斯特菌(Listeria monocytogenes,LM)的污染情况及其分离株生物学特性进行分析,以便为畜禽屠宰和销售环节中LM风险监测提供数据,为防控LM的污染奠定基础。随机采取屠宰场环境、畜禽胴体及污水样品,参照国标方法经增菌、分离,对可疑为LM的菌落进行PCR鉴定,对分离菌株进行血清分型、多位点序列分型(MLST)、生物被膜形成能力、生长曲线和消毒剂最小抑菌浓度(MIC)的分析。结果:1 239份样本检出58株单增李斯特菌,检出率为4.68%,胴体、屠宰环境、屠宰污水在各自样本中检出率分别为4.81%、4.80%、3.48%;58株LM有1/2a(8.62%)、1/2b(44.83%)、1/2c(46.55%)这3种血清型,19种序列型(ST);25株LM分离株生物被膜和生长能力强于参考株;甲醛溶液、苯扎溴铵、碘溶液和氢氧化钠4种消毒剂对7~10株LM分离株的MIC值高于参考株。综上,畜禽屠宰场及农贸市场中存在LM污染,且菌株具有一定的抗逆性。This study was to analyze the contamination of Listeria monocytogenes(LM)in slaughterhouses in Gansu Province and the biological characteristics of LM isolates.Carcasses or environmental samples and sewage samples from slaughterhouses were collected and randomly separated and identified according to National Standard.Then,serotype,multilocus sequence type(MLST),biofilm-forming ability,growth curve and disinfectant minimum inhibitory concentration(MIC)of LM were analyzed.The results showed that there were 58 strains of LM detected in 1239 samples,with a separation rate was 4.68%,and 4.81%(14/291)from carcass and environment,4.80%(40/833)and 3.48%(4/115)in sewage,respectively.The 58 strains of LM showed three serotypes:1/2a(8.62%),1/2b(44.83%),1/2c(46.55%),and 19 ST types.There were 25 LM isolates with higher biofilm and growth ability,compared with those of the standard isolates.However,there were 7-10 LM isolates with higher MIC to formaldehyde solution,benzalammonium bromide,iodine solution and sodium hydroxide to standard strains.There was LM pollution in the livestock slaughterhouses and agricultural products market,and those bacteria possessed certain stress resistance.Therefore,standardizing the disinfection process for the appliances,environment and other aspects in slaughter houses and livestock and poultry products markets and optimizing the use of disinfectants would reduce the risk of human and animal infectionwith LM in the slaughtering process.

关 键 词：单增李斯特菌 多位点序列分型 血清型 生长曲线 消毒剂耐受 

分 类 号：S855[农业科学—临床兽医学]