栀子超高效液相色谱指纹图谱的建立及其降血糖作用谱效关系研究  

Establishment of UPLC fingerprint for Gardeniae Fructus and study on the spectral effect of the hypoglycemic effect

作　　者：商缘君 杜思宇 耿波 陈新 郑国华 胡俊杰 SHANG Yuanjun;DU Siyu;GENG Bo;CHEN Xin;ZHENG Guohua;HU Junjie(College of Pharmacy,Hubei University of Chinese Medicine,Hubei Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《中国医院药学杂志》2025年第2期167-172,共6页Chinese Journal of Hospital Pharmacy

摘　　要：目的:建立栀子的超高效液相色谱(Ultra performance liquid chromatography,UPLC)指纹图谱,研究栀子降血糖的谱效关系,初步明确栀子发挥降血糖作用的物质基础。方法:对11批栀子药材进行UPLC分析,建立指纹图谱,进行相似度评价,结合聚类分析法对指纹图谱数据进行分析;采用UPLC-Q-TOF技术对指纹图谱中的共有特征峰进行鉴定;以α-葡萄糖苷酶抑制率为栀子降血糖的药效指标,研究其降血糖作用;采用灰色关联度分析法和偏最小二乘法回归分析法建立谱效关系,研究栀子降血糖的物质基础。结果:通过指纹图谱的建立,标定了14个共有峰;通过数据库及文献比对初步确定了各特征峰信息;通过测定α-葡萄糖苷酶抑制率,结合谱效关系分析,表明栀子发挥降血糖作用是各种成分共同发挥作用的结果,其中异槲皮素、6″-O-trans-p-cinnamoylgenpin gentiobioside、栀子苷、jasminoside Q、京尼平-1-O-β-D-龙胆双糖苷的贡献程度较大。结论:11批栀子均具有较好的降血糖活性,其降血糖作用是所含成分协同作用的结果,而共有峰7、12、5、8、4所对应的成分与其降血糖活性密切相关,揭示了栀子降血糖活性的药效物质基础。OBJECTIVE To establish the ultra-high performance liquid chromatography(UPLC) fingerprint of Gardeniae Fructus,and to illustrate its spectral effect of the hypoglycemic effect,thus preliminarily clarifying the material basis of the hypoglycemic effect of Gardeniae Fructus.METHODS A total of 11 batches of Gardeniae Fructus were analyzed by UPLC to establish the UPLC fingerprints.Similarity evaluation was carried out,and the fingerprint data were analyzed by clustering analysis.The ultra-high-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to identify the common feature peaks in the fingerprint.The inhibition rate of α-glucosidase was used as the pharmacodynamic index to analyze the hypoglycemic effect of Gardeniae Fructus.The spectral effect of Gardeniae Fructus was established by grey relational analysis(GRA) and partial least squares(PLS) to study the material basis of its hypoglycemic effect.RESULTS Through the establishment of fingerprints,14 common peaks were calibrated.The information of each characteristic peak was preliminarily determined by database searching and literature comparison.The inhibition rate of α-glucosidase was determined by the analysis of spectral activity,showing that the hypoglycemic effect of Gardeniae Fructus was the co-result of various components.Among them,isoquercetin,6″-O-trans-p-cinnamoylgenpin gentiobioside,gardenin,jasminoside Q and genipin-1-O-β-Dgentiobioside contributed greatly.CONCLUSION The 11 batches of Gardeniae Fructus have a good hypoglycemic activity attributed to the synergistic effect of the contained components.The components corresponding to the total peaks 7,12,5,8,4 are closely related to the hypoglycemic activity,revealing the pharmacodynamic material basis of hypoglycemic effect of Gardeniae Fructus.

关 键 词：栀子 指纹图谱 降血糖 谱效关系 

分 类 号：R282[医药卫生—中药学]