铁离子对海洋希瓦氏菌生物被膜诱导厚壳贻贝附着变态能力的影响  

Effects of ferric ions on the capacity of Shewanella marisflavi ECSMB14101 biofilms to induce the settlement and metamorphosis of Mytilus coruscus

作　　者：陶钰 马蕃 彭莉华[1,2,3] 梁箫 杨金龙[1,2,3] TAO Yu;MA Fan;PENG Lihua;LIANG Xiao;YANG Jinlong(Inernational Research Center for Marine Biosciences,Ministry of Seience and Technology,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green culture of Aquaculture Animals,Shanghai Ocean University,Shanghai 201306,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学科技部海洋生物科学国际联合研究中心,上海201306 [2]上海海洋大学水产动物良种创制与绿色养殖协同创新中心,上海201306 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2025年第1期69-79,共11页Journal of Shanghai Ocean University

基　　金：国家重点研发计划(2023YFE0115500,2023YFD2401902)。

摘　　要：为研究铁离子对海洋希瓦氏菌(Shewanella marisflavi ECSMB14101)生物被膜诱导厚壳贻贝(Mytilus coruscus)幼虫附着变态能力的影响,采用不同铁离子浓度培养的单一细菌生物被膜来诱导厚壳贻贝幼虫附着变态,分析了铁离子浓度对海洋希瓦氏菌生物被膜厚壳贻贝幼虫附着变态诱导率、细菌密度、胞外物质以及细胞色素C含量的影响。结果显示,在铁离子浓度分别为0、1、10、20、30、40、50μmol/L的7个实验组中,20μmol/L铁离子浓度培养的海洋希瓦氏菌生物被膜诱导厚壳贻贝幼虫附着变态能力最高,生物被膜细菌密度和细胞色素C等胞外蛋白含量在此浓度下达到最高水平。胞外多糖和胞外脂质不受铁离子浓度的影响。研究表明,在铁离子影响下,海洋希瓦氏菌生物被膜通过调节细胞色素C等胞外蛋白的产生来调控厚壳贻贝幼虫附着变态。本研究可为深入探索厚壳贻贝幼虫附着变态的分子调控机制,以及贝类与细菌相互作用关系的研究奠定坚实的理论基础与参考依据。To study the impact of ferric ions on the ability of Shewanella marisflavi ECSMB14101 biofilms to induce larval settlement and metamorphosis in Mytilus coruscus,single bacterial biofilms cultivated with different ferric ion concentrations were used to induce larval settlement and metamorphosis.The effects of ferric ion concentration on the inducing activity of larval settlement and metamorphosis,bacterial density,extracellular polymeric substances,and cytochrome C content of S.marisflavi biofilms were analysed.Results showed that among the seven groups with ferric ion concentrations of0,1,10,20,30,40,and 50μmol/L,the biofilm of S.marisflavi cultivated at a concentration of 20μmol/L exhibited the highest inducing activity.Furthermore,at this concentration,both the bacterial density of the biofilm and the levels of extracellular proteins and cytochrome C reached their peak among all seven tested concentrations.Extracellular polysaccharides and lipids were not significantly affected by ferric ion concentration.It was shown that under the effects of ferric ions,S.marisflavi biofilms regulate the larval settlement and metamorphosis in M.T.coruscus by modulating the production of extracellular proteins,including cytochrome C.This research offered a solid theoretical foundation and served as a valuable reference for the in-depth investigation of the molecular regulatory mechanisms underlying larval settlement and metamorphosis in M.coruscus.Additionally,it contributes to the understanding of interactions between shellfish and bacteria.

关 键 词：厚壳贻贝 附着变态 海洋希瓦氏菌 铁离子 细胞色素C 

分 类 号：S968.3[农业科学—水产养殖]