利多卡因对结肠癌细胞增殖和迁移的作用及其机制  

Effects and mechanisms of lidocaine on proliferation and migration of colorectal cancer cells

作　　者：罗曼 宋振国[1,2,3,4] LUO Man;SONG Zhenguo(Department of anesthesiology,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China;National Clinical Research Center for Cancer,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China;Tianjin′s Clinical Research Center for Cancer,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China;Key Laboratory of Cancer Prevention and Therapy,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China;Department of Anesthesiology,Tianjin Fifth Central Hospital,Tianjin 300450,China)

机构地区：[1]天津医科大学肿瘤医院麻醉科,天津300060 [2]天津医科大学肿瘤医院国家肿瘤临床医学研究中心,天津300060 [3]天津医科大学肿瘤医院天津市恶性肿瘤临床医学研究中心 [4]天津医科大学肿瘤医院天津市“肿瘤防治”重点实验室,天津300060 [5]天津市第五中心医院麻醉科,天津300450

出　　处：《天津师范大学学报(自然科学版)》2025年第1期22-26,共5页Journal of Tianjin Normal University:Natural Science Edition

基　　金：天津市医学重点学科(专科)建设项目(TJYXZDXK-009A).

摘　　要：为了探究麻醉剂利多卡因对结肠癌的治疗作用及机制,选取人结肠癌细胞系LOVO和HCT116进行分组实验.用2 mmol/L的利多卡因(0 mmol/L为对照组)处理LOVO和HCT116细胞24 h,用CCK-8和克隆形成实验评估细胞生长,用Transwell和划痕实验评估细胞运动能力,用免疫印迹和铁检测试剂盒评估铁死亡,并通过免疫印迹实验评估PI3K/Akt/mTOR信号通路的激活.结果显示:①同对照组相比,利多卡因能够显著抑制人结肠癌细胞的生长和移动性;②利多卡因在结肠癌细胞中能够促进铁死亡;③利多卡因的抗肿瘤效应与抑制PI3K/Akt/mTOR信号通路有关,该通路对细胞生存和生长至关重要.总之,利多卡因可通过抑制结肠癌细胞生长和运动、促进铁死亡和抑制PI3K/Akt/mTOR信号通路,对结肠癌产生一定的治疗效果.To investigate the therapeutic effects and mechanism of lidocaine on colorectal cancer,the human colorectal cancer cell line LOVO and HCT116 were selected for grouped experiments.LOVO and HCT116 cells were treated with 0 mmol/L and 2 mmol/L concentrations of lidocaine for 24 h.The cell growth was evaluated using CCK-8 and clonogenic assays,while cell motility was assessed through Transwell and wound healing assays.Ferroptosis was measured using immunoblotting and iron detection kits,and the activation of PI3K/Akt/mTOR signaling pathway was evaluated by immunoblotting.The results showed that:①Lidocaine significantly inhibited the growth and motility of human colorectal cancer cells compared with control group;②Lidocaine promoted ferroptosis in colorectal cancer cells;③The antitumor effects of lidocaine were associated with the inhibition of PI3K/Akt/mTOR signaling pathway,which is crucial for cell survival and growth.Overall,lidocaine exerts antitu-mor effects on colorectal cancer cells by inhibiting cell growth and motility,promoting ferroptosis,and suppressing the PI3K/Akt/mTOR signaling pathway.

关 键 词：结肠癌 利多卡因 细胞生长 铁死亡 PI3K/Akt/mTOR信号通路 

分 类 号：R735.35[医药卫生—肿瘤]