不同电子供体底物对自养型硫氧化菌同步厌氧除硫脱氮过程的影响  

Influence of different electron donor substrates on simultaneous anaerobic sulfide and nitrate removal of autotrophic sulfur-oxidizing bacteria

作　　者：吴新世[1] 朱广斌 张万良 WU Xinshi;ZHU Guangbin;ZHANG Wanliang(School of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384,China;Tianjin Tasly Pharmaceu-tical Group Co.,Ltd.,Tianjin 300410,China)

机构地区：[1]天津理工大学化学化工学院,天津300384 [2]天津天士力医药集团股份公司,天津300410

出　　处：《天津师范大学学报(自然科学版)》2025年第1期34-43,共10页Journal of Tianjin Normal University:Natural Science Edition

基　　金：高校产学研联合创新项目(H20220409).

摘　　要：采用分段递进式废水除硫工艺(SPWSRP)研究不同电子供体底物对自养型硫氧化菌同步厌氧除硫脱氮过程的影响.控制不同反应体系的硫氮比(S/N)和pH值,可使反应朝着预定方向进行.以S^(2-)为电子供体底物时,设定硫氮比为3∶2和pH值为6.9±0.1,S^(2-)和NO_(3)^(-)对应的最大去除率分别为99.5%和99.9%,对应的最大容积载荷分别为4.32 kg/(m^(3)·d)和1.11 kg/(m^(3)·d);代谢途径长且复杂多样化,相应代谢产物种类也呈多样性,包括多种中间产物如S0、S_(2)O_(3)^(2-)、NO_(2)^(-)和终产物SO_(4)^(2-)、N_(2);同时对实验过程中的典型现象如铵盐出水浓度变化、反应停滞机理等进行了分析.以S_(2)O_(3)^(2-)为电子供体底物时,设定硫氮比为5:8和pH值为7.1±0.1,S_(2)O_(3)^(2-)和NO_(3)^(-)对应的最大去除率分别为99.8%和99.9%,最大容积载荷分别为8.66 kg/(m^(3)·d)和3.04 kg/(m^(3)·d);代谢途径短且简单,生成的中间产物和终产物相对较少,中间产物仅检测有NO_(2)^(-),终产物则包括SO_(4)^(2-)、N_(2)等,单质硫(S0)或其他氮氧类化合物未检出.对比发现,以S_(2)O_(3)^(2-)为底物的代谢产物与以S^(2-)为底物的产物既有相似性,又有很大差异.电子供体不同的两种体系反应路径不同、反应机理不同、反应停滞原因也不同.从代谢过程看,S_(2)O_(3)^(2-)代谢途径并非S^(2-)代谢途径的缩短化.基于此,废水处理中选择合适的电子供体底物,既能达到除硫脱氮目的,又能防止副反应的发生.The influence of different electron donor substrates on simultaneous anaerobic sulfide and nitrate removal of auto-trophic sulfur-oxidizing bacteria was studied using a segmented progressive wastewater sulfide removal process(SPWSRP).In the experiment,if the ratio of sulfide to nitrate(S/N)and pH value of the wastewater system were controlled well,the system reac-tion was predictable.When sulfide was used as the electron donor substrate,and if the S/N ratio was set at 3∶2 and the influent pH value at 6.9±0.1,the maximum removal percentages of sulfide and nitrate in the wastewater were 99.5%and 99.9%respectively,and their corresponding maximum volumetric removal load rates were 4.32 kg/(m^(3)·d)and 1.11 kg/(m^(3)·d)respectively.The substrates′metabolic pathways were relatively long and complicated,and the corresponding types of metabolites were diverse.Then the metabolites covered several metabolic intermediates such as S0,S_(2)O_(3)^(2-)and NO_(2)^(-)and end products including SO_(4)^(2-)and N_(2).In addition,the possible reaction mechanisms were analyzed based on typical experimental phenomena such as reaction cessation,and variation of ammonium concentration in the wastewater.When thiosulfate was used as the electron donor sub-strate,and the S/N ratio was set at 5∶8 and the influent pH value at 7.1±0.1,the maximum removal efficiencies of thiosulfate and nitrate in the reaction system were separately 99.8%and 99.9%,and their respective maximum volumetric loads were 8.66 kg/(m^(3)·d)and 3.04 kg/(m^(3)·d)respectively.The substrates′metabolic pathway was relatively short and simple,with rela tively few intermediates and end products being generated.The detectable intermediate was only NO_(2)^(-),and the end products included SO_(4)^(2-)and N_(2).While elemental sulfur and other nitrogen-oxides were not detected.The metabolites including intermediates and end products were similar to those with sulfide as electron donor substrate,but with huge differences.Two systems with different electron donor as su

关 键 词：同步厌氧除硫脱氮 废水除硫工艺 电子供体底物 硫氮比 代谢途径 代谢产物 

分 类 号：Q939.9[生物学—微生物学] X172[环境科学与工程—环境科学]