海南岛2种森林类型地下水微生物研究  

Microorganism of Groundwater in Two Forest Types in Hainan Island

作　　者：庞通 孙瑞[1,2] 兰国玉 魏亚情[1,2] 杨川 符庆茂 PANG Tong;SUN Rui;LAN Guoyu;WEI Yaqing;YANG Chuan;FU Qingmao(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China;Danzhou Investigation&Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,Danzhou 571737,Hainan,China;School of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao 266109,Shandong,China)

机构地区：[1]中国热带农业科学院橡胶研究所,海南海口571101 [2]海南儋州热带农业生态系统国家野外科学观测研究站,海南儋州571737 [3]青岛农业大学园林与林学院,山东青岛266109

出　　处：《西北林学院学报》2025年第2期93-102,共10页Journal of Northwest Forestry University

基　　金：国家自然科学基金项目(32271603);海南省自然科学基金高层次人才项目(320RC733);中央级公益性科研院所基本科研业务费专项(1630022022003)。

摘　　要：探究不同森林地下水微生物群落组成和多样性及环境驱动因素,为热带森林研究提供基础数据。本研究利用宏基因组测序技术分析了海南岛热带雨林和橡胶林2种森林的地下水微生物群落组成、多样性以及环境因素。结果表明:1)热带雨林与橡胶林地下水微生物群落构成存在明显差异,古菌群落方面,橡胶林奇古菌门(Thaumarchaeota)占比极显著高于热带雨林(P<0.001),而广古菌门(Euryarchaeota)则显著低于热带雨林(P<0.05);细菌群落中,橡胶林变形菌门(Proteobacteria)略高于热带雨林,而拟杆菌门(Bacteroidetes)则低于热带雨林(P<0.05);真菌群落中橡胶林子囊菌门(Ascomycota)显著高于热带雨林(P<0.05),而毛霉菌门(Mucoromycota)极显著低于热带雨林(P<0.001)。2)热带雨林的地下水微生物α多样性高于橡胶林,从热带雨林到橡胶林古菌群落的α多样性极显著降低(P<0.001),细菌群落的α多样性显著降低(P<0.05),真菌群落的α多样性显著升高(P<0.05)。主坐标分析(PCoA)和相似性分析揭示,海南岛橡胶林和热带雨林地下水微生物β多样性具有极显著差异(古菌:R=0.666,P=0.001;细菌:R=0.519,P=0.001;真菌:R=0.384,P=0.001)。3)地下水的溶解性总固体、海拔和氧化还原电位分别是影响古菌群落、细菌群落和真菌群落最重要的环境因子,大气压、重碳酸盐和海拔对地下水微生物β多样性具有显著影响,其中海拔是最主要的环境因素。The objective of this study was to explore the composition and diversity of groundwater microbial communities and environmental driving factors in different forests,so as to provide basic data for the study of tropical forests.Metagenomic sequencing technology was used to analyze the composition and diversity of groundwater microbial communities and environmental factors in two types of forests,tropical rainforest and rubber forest occurring in Hainan Island.The results showed that 1)there were obvious differences in the composition of groundwater microbial communities between tropical rainforest and rubber forest(P<0.001),and the proportion of archaeal community was higher than that of tropical rainforest,while that of Euryarchaeota was significantly lower than that of tropical rainforest(P<0.05).Among the bacterial communities,Proteobacteria was slightly higher in rubber forest than in tropical rainforest,while Bacteroidetes was lower than that in tropical rainforest(P<0.05).In the fungal community,Ascomycota was significantly higher than that in tropical rainforest(P<0.05),while Mucoromycota was significantly lower than in tropical rainforest(P<0.001).2)Theαdiversity of groundwater microorganisms in tropical rainforests was higher than that in rubber forests.Theαdiversity of archaeal communities from tropical rainforests to rubber forests significantly reduced(P<0.001),theαdiversity of bacterial communities significantly reduced(P<0.05),and theαdiversity of fungal communities significantly increased(P<0.05).Principal coordinate analysis(PCoA)and similarity analysis revealed theβdiversity of groundwater microorganisms,significant differences were observed between rubber forests and tropical rainforests in Hainan Island(archaea:R=0.666,P=0.001;bacteria:R=0.519,P=0.001;fungi:R=0.384,P=0.001).3)The total dissolved solids,altitude,and redox potential of groundwater were the most important environmental factors affecting archaeal,bacterial,and fungal communities,respectively.Atmospheric pressure,bicarbonate,and

关 键 词：热带森林 地下水 微生物多样性 环境因子 

分 类 号：S718.521.3[农业科学—林学]