五味子乙素对胰腺癌细胞迁移和侵袭的抑制作用及其机制  

Inhibitory effect of schisandrin on migration and invasion of pancreatic cancer cells and its mechanism

作　　者：杨露 傅家财 李凤金 齐玲 YANG Lu;FU Jiacai;LI Fengjin;QI Ling(School of Pharmacy,Zunyi Medical University,Zunyi 563000,China;Department of Gastroenterology,People’s Hospital,Qingyuan City,Guangdong Province,Affiliated Qingyuan Hospital,Guangzhou Medical University,Qingyuan 511500,China)

机构地区：[1]遵义医科大学药学院,贵州遵义563000 [2]广州医科大学附属清远医院广东省清远市人民医院消化内科,广东清远511500

出　　处：《吉林大学学报(医学版)》2025年第1期44-50,共7页Journal of Jilin University:Medicine Edition

基　　金：国家自然科学基金项目(82203351);广东省中医药局科研项目(20231412,20211458);广东省卫健委医学科学技术研究基金项目(A2022163)。

摘　　要：目的:探讨五味子乙素(SchB)对胰腺癌Pan02细胞迁移和侵袭的抑制作用,并阐明其相关作用机制。方法:胰腺癌Pan02细胞经不同浓度(0、0.78、1.56、3.12、6.25、12.50和25.00 mg·L^(-1))SchB处理24、48和72 h后,采用CCK-8法检测各组细胞存活率,确定后续实验SchB用药浓度。胰腺癌Pan02细胞分为对照组和2.5、5.0及10.0 mg·L^(-1)SchB组。细胞划痕实验检测各组胰腺癌Pan02细胞划痕愈合率,Transwell小室实验检测各组胰腺癌Pan02细胞迁移和侵袭细胞数,Western blotting法检测各组胰腺癌Pan02细胞中波形蛋白(Vimentin)和N钙黏蛋白(N-cadherin)表达水平。构建胰腺癌Pan02细胞小鼠皮下移植瘤模型,成功建模的10只小鼠随机分为对照组和SchB组,每组5只;处理28 d后,测量小鼠肿瘤质量,计算瘤体积;采用免疫组织化学法检测各组小鼠肿瘤组织中Vimentin和N-cadherin蛋白表达情况。结果:CCK-8法,与对照组比较,其他浓度SchB组胰腺癌Pan02细胞存活率降低(P<0.05或P<0.01);细胞划痕实验,与对照组比较,2.5、5.0和10.0 mg·L^(-1)SchB组胰腺癌Pan02细胞划痕愈合率降低(P<0.05或P<0.01);Transwell小室实验,与对照组比较,2.5、5.0和10.0 mg·L^(-1)SchB组胰腺癌Pan02细胞迁移和侵袭细胞数减少(P<0.05或P<0.01);Western blotting法,与对照组比较,2.5、5.0和10.0 mg·L^(-1)SchB组胰腺癌Pan02细胞中Vimentin和N-cadherin蛋白表达水平降低(P<0.05或P<0.01)。与对照组比较,SchB组小鼠肿瘤体积和质量均明显降低(P<0.01)。免疫组织化学染色,与对照组比较,SchB组小鼠肿瘤组织中Vimentin和N-cadherin蛋白阳性表达率明显降低(P<0.01)。结论:SchB能够抑制胰腺癌Pan02细胞的增殖、迁移和侵袭能力,其作用机制与降低Vimentinh和N-cadherin蛋白表达有关。Objective:To discuss the inhibitory effect of schisandrin B(SchB)on the migration and invasion of the pancreatic cancer Pan02 cells,and to clarify its mechanism.Methods:The pancreatic cancer Pan02 cells were treated with different concentrations of SchB(0,0.78,1.56,3.12,6.25,12.50,and 25.00 mg·L^(-1))for 24,48,and 72 h.CCK-8 method was used to detect the survival rates of the cells in various groups,and the concentration of SchB for the subsequent experiments was confirmed.The Pan02 cells were divided into control group,2.5 mg·L^(-1)SchB group,5.0 mg·L^(-1)SchB group,and 10.0 mg·L^(-1)SchB group.Wound healing assay was used to detect the wound healing rates of the Pan02 cells in various groups;Transwell chamber assay was used to detect the numbers of migration and invasion Pan02 cells in various groups;Western blotting method was used to detect the expression levels of Vimentin and N-cadherin proteins in the Pan02 cells in various groups.The mouse models of subcutameous transplanted tumor of pancreatic cancer cells were established.Ten successfully modeling mice were randomly divided into control group and SchB group(n=5).After 28 d of treatment,the weights of tumor of the mice were determined;immunohistochemistry method was used to detect the expressions of Vimentin and N-cadherin proteins in tumor tissue of the mice in various groups.Results:The CCK-8 results showed that compared with control group,the survival rates of the pancreatic cancer Pan02 cells in different concentrations of SchB groups were decreased(P<0.05 or P<0.01).The wound healing results showed that compared with control group,the wound healing rates of the cells in 2.5,5.0,and 10.0 mg·L^(-1)SchB groups were decreased(P<0.05 or P<0.01).The Transwell chamber results showed that compared with control group,the numbers of migration and invasion Pan02 cells in 2.5,5.0,and 10.0 mg·L^(-1)SchB groups were decreased(P<0.05 or P<0.01).The Western blotting results showed that compared with control group,the expression levels of Vimentin and N-cadher

关 键 词：五味子乙素 胰腺肿瘤 细胞迁移 细胞侵袭 N钙黏蛋白 波形蛋白 

分 类 号：R735.9[医药卫生—肿瘤]