心理应激对SD大鼠颞下颌关节髁突软骨细胞凋亡及Caspase-3表达的影响  

The Effects of Psychological Stress on Apoptosis of Condylar Chondrocytes in Temporomandibular Joint and Expression of Caspase-3 in Rats

作　　者：郑程东 张媛媛 张海娟[1,2,3] 韩妍冬 于传垒 陈鹤玲 王爽[1,2,3] ZHENG Chengdong;ZHANG Yuanyuan;ZHANG Haijuan;HAN Yandong;YU Chuanlei;CHEN Heling;WANG Shuang(Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi'an Jiaotong University,Xi'an 710004,Shaanxi,China;Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases,College of Stomatology,Xi'an Jiaotong University,Xi'an 710004,Shaanxi,China;Department of Orthodontics,College of Stomatology,Xi'an Jiaotong University,Xi'an 710004,Shaanxi,China;ClearMed Technology Co.,Ltd,Changzhou 213145,Jiangsu,China)

机构地区：[1]西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室,陕西西安710004 [2]西安交通大学口腔医院陕西省牙颌面疾病临床研究中心,陕西西安710004 [3]西安交通大学口腔医院口腔正畸科,陕西西安710004 [4]可丽尔医疗科技常州有限公司,江苏常州213145

出　　处：《中国美容医学》2025年第3期7-13,共7页Chinese Journal of Aesthetic Medicine

摘　　要：目的:本研究旨在探讨单纯心理应激对大鼠颞下颌关节髁突软骨细胞凋亡及Caspase-3表达的影响。方法:通过建立大鼠的单纯心理应激动物模型,并采用HE染色、TUNEL法和免疫组织化学等实验方法观察不同时间点下大鼠颞下颌关节软骨的组织学变化、软骨细胞凋亡情况以及Caspase-3在软骨中的表达变化。结果:与对照组相比,在心理应激1周、3周和5周后,实验组大鼠出现显著的行为学改变,体重增长量略有减少但差异无统计学意义(P>0.05);旷场实验和高架实验表明心理应激组大鼠的焦虑水平增高(P<0.05);组织学分析发现,心理应激导致软骨结构损伤,软骨细胞凋亡率增加,且随应激时间延长而加剧(P<0.05);Caspase-3的表达也随应激时间的延长而增加,但在恢复期有所下降(P<0.05)。结论:单纯心理应激可引起颞下颌关节髁突软骨病理性改变,可能与关节软骨内Caspase-3表达增加及软骨细胞过度凋亡有关。Objective This study aimed to explore the effects of simple psychological stress on the apoptosis of condylar chondrocytes and the expression of Caspase-3 in rats.Methods The histological changes in rat TMJ cartilage,chondrocyte apoptosis,and Caspase-3 expression post-stress will be examined using a psychological stress model in rats with HE staining,TUNEL assay,and immunohistochemistry.Results The results showed that compared with the control group,significant behavioral changes were observed in the experimental group rats after 1 week,3 weeks,and 5 weeks of psychological stress(P>0.05).There was a slight reduction in body weight gain but no statistical difference,open field and elevated tests indicated that the anxiety level of rats in the psychological stress group increased(P<0.05).Histological analysis found that psychological stress led to damage of cartilage structure and increase in chondrocyte apoptosis rate,which worsened with the extension of stress time(P<0.05).The expression of Caspase-3 also increased with the extension of stress time,but decreased in the recovery period(P<0.05).Conclusion This indicates that simple psychological stress can cause pathological changes in the condylar cartilage of the temporomandibular joint,which may be related to increased expression of Caspase-3 in the articular cartilage and excessive apoptosis of chondrocytes.

关 键 词：心理应激 颞下颌关节 颞下颌关节紊乱病 髁突软骨 细胞凋亡 Caspase-3 

分 类 号：R782.6[医药卫生—口腔医学]