积雪草不同溶剂提取物的抗氧化活性及其稳定性研究  

Study on antioxidant activity and stability of Centella asiatica extracts over different solvents

作　　者：关富强 彭效明[1] 居瑞军[1] 杨思敏 严晓强 管洁 GUAN Fu-qiang;PENG Xiao-ming;JU Rui-jun;YANG Si-min;YAN Xiao-qiang;GUAN Jie(Beijing Key Laboratory of Enze Biomass Fine Chemicals,Beijing Institute of Petrochemical Technology,Beijing 102617,China)

机构地区：[1]北京石油化工学院,北京市恩泽生物质重点实验室,北京102617

出　　处：《现代化工》2025年第3期100-105,共6页Modern Chemical Industry

基　　金：国家自然科学基金项目(21406015);北京市教委科技计划一般项目(KM202210017011,KM202310017009);北京石油化工学院校内学科平台建设项目(2019XK006);北京石油化工学院交叉科研探索项目(BIPTCSF-2023008);北京石油化工学院致远科研基金(2023013);北京市科协青年人才托举工程项目(BYESS2023259);大学生创新创业训练计划项目(2024J00293,2024J00276)。

摘　　要：利用不同质量分数的乙醇以及水作为溶剂,系统比较了不同溶剂提取物中多酚的质量分数及其对DPPH和ABTS自由基的清除能力。结果表明,80%乙醇提取物中多酚质量分数最高(9.28 mg/g),其抗氧化活性在所有溶剂中表现最佳,DPPH清除率高达96.23%,ABTS清除率达95.28%,表明多酚质量分数与抗氧化能力之间存在强正相关。此外,探讨了光照、温度、pH和特定化学物质对多酚稳定性的影响,结果发现高温和强光照显著降低多酚稳定性,而适宜的酸性环境可显著提高其稳定性。同时优化了积雪草多酚的提取条件,为其在医药和化妆品行业中的应用提供了新的科学依据。The mass fractions of polyphenols in the extracts from Centella asiatica by different solvents that contain ethanol and water with varying mass fractions from 20%to 95%,and their scavenging abilities against DPPH and ABTS free radicals are systematically compared.Results show that the extract by 80%ethanol has the highest polyphenol mass fraction(9.28 mg·g-1),and the best antioxidant activity.The clearance rates of DPPH and ABTS by it are as high as 96.23%and 95.28%,respectively,which also indicates there is a strong positive correlation between polyphenols content and antioxidant capacity.In addition,the impacts of illumination,temperature,pH and specific chemicals on the stability of polyphenols are explored.It is found that high temperature and strong illumination can significantly reduce the stability of polyphenols,while a suitable acidic environment can significantly improve its stability.The conditions for the extraction of polyphenols from Centella asiatica are optimized,providing new scientific basis for the application of Centella asiatica polyphenols in the pharmaceutical and cosmetics industries.

关 键 词：积雪草 多酚 极性部位 抗氧化 稳定性 

分 类 号：O657.3[理学—分析化学]