阿纳其根醇提取物调节肠道菌群改善高脂饮食诱导小鼠血糖紊乱的实验研究  

Experimental Study on Ethanol Extract of the Root of Anacyclus Pyrethrum Regulates Gut Microbiota to Improve High-fat Diet-induced Dysglycemia in Mice

作　　者：杨浩 古丽若依·帕尔哈提[3] 金小越 YANG Hao;Guliruoyi·Paerhati;JIN Xiaoyue(State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,Xinjiang 830011,China)

机构地区：[1]省部共建中亚高发病成因与防治国家重点实验室,乌鲁木齐830011 [2]新疆医科大学第六附属医院药学部(第六临床医学院药学教研室),乌鲁木齐830000 [3]新疆医科大学药学院,乌鲁木齐830017

出　　处：《医学研究杂志》2025年第1期106-110,共5页Journal of Medical Research

基　　金：省部共建中亚高发病成因与防治国家重点实验室开放课题资助项目(SKL-HIDCA-2022-28)。

摘　　要：目的 研究阿纳其根醇提取物(ethanol extract of the root of anacyclus pyrethrum,EEAP)对高脂饮食小鼠血糖及其对肠道菌群的影响。方法 将48只C57BL/6N雄性小鼠按照随机数字表法分为对照组,模型组,低、中、高剂量EEAP组及二甲双胍组,每组各8只。低、中、高剂量EEAP组小鼠分别给予100、200、400mg/kg的EEAP,二甲双胍组小鼠给予二甲双胍280mg/kg,对照组和模型组均给予等量蒸馏水,每天灌胃1次,连续12周。除对照组小鼠饮食采用正常饲料外,其余各组小鼠均采用高脂饲料。使用血糖试纸检测各组小鼠空腹和随机血糖,采用酶联免疫吸附试验试剂盒检测小鼠炎性细胞因子水平,通过16S rDNA实时荧光定量聚合酶链反应检测肠道菌群。结果 在第12周时,与模型组比较,低、中、高剂量EEAP组和二甲双胍组小鼠体质量均明显下降。与模型组比较,高剂量EEAP组和二甲双胍组小鼠空腹血糖和随机血糖明显降低。与模型组比较,中、高剂量EEAP组和二甲双胍组小鼠肠道中嗜黏蛋白阿克曼菌和普拉梭菌丰度明显升高,而脱硫弧菌丰度明显降低。结论 EEAP可改善高脂饮食诱导小鼠血糖紊乱,其机制可能与调节肠道菌群、抑制炎性反应有关。Objective To study the effects of ethanol extract of the root of anacyclus pyrethrum(EEAP) on blood glucose and gut microbiota in mice induced by high-fat diet.Methods Forty-eight male C57BL/6N mice were randomly allocated into groups using a random number table:control group,model group,low-,medium-,and high-dose EEAP groups,as well as a metformin group,with each group consisting of 8mice.Mice in the low,medium,and high dose EEAP groups were administered EEAP at 100,200 and 400mg/kg,respectively.The metformin group was given metformin at 280mg/kg.Both the control and model groups were given an equivalent amount of distilled water.Gavage was performed once daily for 12 consecutive weeks.Except the control group mice were fed normal diet,the other groups were fed high-fat diet.Fasting and random blood glucose were detected by blood glucose test strips.The levels of inflammatory factors in mice were detected by enzyme-linked immunosorbent assay kit.Gut microbiota was detected by 16S rDNA real-time quantitative polymerase chain reaction.Results At 12th weeks,compared with the model group,the body mass of mice in low-dose,medium-dose and high-dose EEAP groups and metformin group were decreased significantly.Compared with the model group,the fasting blood glucose and random blood glucose in high-dose EEAP group and metformin group were significantly decreased.Compared with the model group,the abundations of Akkermansia muciniphila and Faecalibacterium prausnitzii in the intestinal tracts of mice in medium-dose and high-dose EEAP groups and metformin group were significantly increased,while the abundations of desulfovibrio were significantly decreased.Conclusion EEAP can improve dysglycemia induced by high-fat diet in mice,and its mechanism may be related to regulating gut microbiota and inhibiting inflammatory reaction.

关 键 词：阿纳其根醇提取物 肠道菌群 高脂饮食 肥胖 血糖紊乱 

分 类 号：R285.5[医药卫生—中药学]