Zeb1在小鼠实验性根尖周炎中的表达及作用研究  

Expression of Zeb1 in mice apical periodontitis and its possible mechanism

作　　者：马子涵 林莹 王紫君 孙筱月 朱玲新 MA Zi-han;LIN Ying;WANG Zi-jun;SUN Xiao-yue;ZHU Ling-xin(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,Key Laboratory of Oral Biomedicine Ministry of Education,Hubei Key Laboratory of Stomatology,School&Hospital of Stomatology,Wuhan University,Hubei Wuhan 430079,China;Department of Stomatology,Shenzhen Children's Hospital,Guangdong Shenzhen 518000,China)

机构地区：[1]口颌系统重建与再生全国重点实验室,口腔生物医学教育部重点实验室,口腔医学湖北省重点实验室,武汉大学口腔医(学)院,湖北北武汉430079 [2]深圳市儿童医院口腔科,广东深圳518000

出　　处：《临床口腔医学杂志》2025年第2期67-71,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金项目(编号:82370914、81970919、82201042);中央高校基本科研业务费专项资金(编号:2042024YXA010)。

摘　　要：目的:研究锌指E盒结合蛋白1(zinc finger E-box-binding protein 1,Zeb1)在小鼠根尖周炎病损中的表达及其调控作用。方法:通过小鼠下颌第一磨牙牙髓暴露构建小鼠根尖周炎模型,术后0、14、28 d获取下颌骨,苏木精-伊红(hematoxylin-eosin staining,H&E)染色及TRAP染色观察根尖周病变的炎症浸润以及破骨细胞的形成情况,高分辨率X线、显微CT扫描观察根尖周骨破坏的严重程度。免疫荧光双重染色检测Zeb1在根尖周炎发生发展过程中的表达以及其与巨噬细胞的共定位。体外提取分离野生型及髓系Zeb1条件性敲除小鼠的骨髓来源巨噬细胞进行迁移功能和吞噬功能检测。结果:随着时间的推移,根尖周病损的炎症范围扩大,破骨细胞数目增多,根尖周骨吸收加重。Zeb1的表达随着根尖周病变范围的扩大而迅速增加,同时Zeb1阳性细胞的分布与巨噬细胞明显重叠。体外实验显示在LPS的刺激下,Zeb1条件性敲除巨噬细胞的吞噬能力明显降低(P<0.05),而迁移能力无变化。结论:Zeb1的表达与根尖周炎病变大小呈显著正相关,可能通过调控巨噬细胞的吞噬功能参与根尖周炎的发生发展。Objective:To investigate the expression and effect of zinc finger E-box-binding protein 1(Zeb1)in mice apical periodontitis.Methods:Apical periodontitis model was induced by exposing the pulp of the mandibular first molars in mice and the mandibles were collected on 0,14,28 days after pulp exposure.H&E and TRAP staining were performed to observe inflammatory infiltration and osteoclast formation in periapical lesions,while micro-CT and high-resolution X-ray imaging were used to assess the extent of periapical bone destruction.Zeb1 expression in periapical inflammation and its co-localization with macrophages were detected by immunofluorescence double staining.Bone marrow-derived macrophages from wild-type and Zeb1 conditional knockout mice were isolated in vitro to detect their migration and phagocytic functions.Results:A significant increase in inflammatory cells infiltration,osteoclasts formation in periapical lesion,and periapical bone resorption was observed over time following pulp exposure.As the lesion expanded,Zeb1 expression increased rapidly,showing a strong co-localization between the distribution of Zeb1-positive cells and macrophages.Additionally,in vitro experiments revealed a substantial reduction in the phagocytic capacity of Zeb1 conditional knockout macrophages with LPS stimulation(P<0.05),while their migration ability remained unaffected.Conclusion:Zeb1 expression was significantly and positively correlated with the severity of periapical inflammation,suggesting its involvement in the progression of periapical inflammation through the regulation of macrophage phagocytosis.

关 键 词：锌指E盒结合蛋白1(Zeb1) 巨噬细胞 吞噬功能 根尖周炎 

分 类 号：R781.3[医药卫生—口腔医学]