槲皮素对小鼠肌内脂肪沉积的影响及其机制探究  

Effects of Quercetin on Intramuscular Fat Deposition in Mice and Its Mechanisms

作　　者：洪妍 刘佳琪 杨童寓丹 王新霞 HONG Yan;LIU Jiaqi;YANG Tongyudan;WANG Xinxia(College of Animal Science,Zhejiang University,Hangzhou 310058,China;Key Laboratory of Molecular Animal Nutrition(Zhejiang University),Ministry of Education,Hangzhou 310058,China;Key Laboratory of Animal Nutrition and Feed Science(Eastern of China),Ministry of Agriculture and Rural Affairs,Hangzhou 310058,China)

机构地区：[1]浙江大学动物科学学院,杭州310058 [2]浙江大学动物分子营养学教育部重点实验室,杭州310058 [3]农业农村部动物营养与饲料(华东)重点实验室,杭州310058

出　　处：《动物营养学报》2025年第2期1298-1311,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金重点项目(32330098)。

摘　　要：本试验旨在探究槲皮素对小鼠肌内脂肪沉积的影响及其潜在调节机制。活体试验选取24只7周龄雄性C57BL/6J小鼠,预试7 d后随机平均分为2组,即对照组(NCD组)和槲皮素组(NQ组),每组6个重复,每个重复2只小鼠。NCD组小鼠每日灌胃10μL/g BW的磷酸盐缓冲液(PBS),NQ组小鼠每日灌胃10μL/g BW的槲皮素溶液(10 mg/mL)。试验期42 d,期间所有小鼠均饲喂基础饲粮,自由采食和饮水。试验进行至第29天,向小鼠双侧胫骨前肌注射50%甘油以诱导构建肌肉脂肪浸润模型,并分别于造模后第7和14天取样。细胞试验选取8周龄雄性C57BL/6J小鼠,分离并培养小鼠成纤维/成脂祖细胞(FAPs),梯度槲皮素(0、1、5、10、20和40μmol/L)处理,采用鸡尾酒法诱导细胞成脂分化。结果表明:1)100 mg/kg槲皮素抑制小鼠肌内脂肪沉积,但对小鼠体重、采食量和血清甘油三酯含量无显著影响(P>0.05)。2)槲皮素呈剂量依赖性抑制小鼠FAPs分化聚酯。3)槲皮素影响小鼠粪便菌群结构,降低厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)等的相对丰度以及厚壁菌门/拟杆菌门值,提高罗姆布茨菌属(Romboutsia)和回肠杆菌属(Ileibacterium)等的相对丰度。4)槲皮素影响小鼠粪便代谢物,差异代谢物富集在氨基酸、脂肪酸和胆汁酸代谢相关通路;其中,与NCD组相比,NQ组粪便精胺、亚精胺和肌苷等代谢物含量显著提高(P<0.05),粪便二十碳五烯酸(EPA)和20-羟基二十碳四烯酸(20-HETE)等代谢物含量显著降低(P<0.05)。5)槲皮素显著提高小鼠胫骨前肌mRNA N^(6)-甲基腺苷(m^(6)A)水平(P<0.05)。综上所述,槲皮素可以显著抑制小鼠肌内脂肪沉积,可能是通过改变小鼠肠道微生物群落结构和相关代谢产物以及肌肉组织m^(6)A甲基化修饰等途径发挥调节作用。The experiment was conducted to investigate the effects of quercetin on intramuscular fat(IMF)deposition in mice and its potential regulatory mechanisms.Twenty-four 7-week-old male C57BL/6J mice were selected and randomly divided into two groups as control group(NCD group)and quercetin group(NQ group)with 6 replicates in each group and 2 mice in each replicate.Mice in NCD group were gavaged with 10μL/g BW phosphate buffer solution(PBS)daily,while those in NQ group were gavaged with 10μL/g BW quercetin solution(10 mg/mL)daily.The experimental period lasted for 42 days,during which all mice were freely fed a basic diet and drinking water.On the 29th day of the experiment,50% glycerol was injected into the bilateral tibialis anterior of mice to induce the establishment of muscle fat infiltration model,and muscle samples were taken on the 7th and 14th day after the modeling.Male C57BL/6J mice aged at 8 weeks were selected for cell experiment,and mouse fibro/adipogenic progenitors(FAPs)were isolated and cultured.Cocktail method was used to induce adipogenic differentiation while treating with gradient quercetin(0,1,5,10,20 and 40μmol/L).The results showed as follows:1)100 mg/kg BW quercetin inhibited IMF deposition in mice,but had no significant differences on mice weight,feed intake,and serum triglyceride content(P>0.05).2)Quercetin dose-dependently inhibited FAPs differentiation polyester.3)Quercetin altered the fecal microbiota structure of mice,reduced the relative abundances of Firmicutes and Bacteroidota as well as Firmicutes to Bacteroidota ratio,while increased the relative abundances of Romboutsia and Ileibacterium.4)Quercetin altered the fecal metabolites of mice,and the differential metabolites were enriched in pathways related to amino acid,fatty acid and bile acid metabolism.Among them,compared with NCD group,the fecal contents of metabolites such as spermine,spermidine and inosine in NQ group were significantly increased(P<0.05),while the fecal contents of metabolites such as eicosapentaenoic acid(EPA

关 键 词：槲皮素 小鼠 肌内脂肪 肠道微生物 代谢物 m^(6)A 

分 类 号：S811[农业科学—畜牧学]