羊源卷曲乳杆菌DY38的体外益生特性及抗氧化活性研究  

In Vitro Probiotic Properties and Antioxidant Activity of Lactobacillus crispatus DY38 from Goats

作　　者：肖春进 岳秀英[2] 吴晓岚[2] 叶忠明 杨发龙[1] XIAO Chunjin;YUE Xiuying;WU Xiaolan;YE Zhongming;YANG Falong(College of Animal Husbandry and Veterinary Medicine,Southwest Minzu University,Chengdu 610041,China;Sichuan Veterinary Drug Inspection Institute,Chengdu 610041,China;Animal Husbandry Science Research Institute of Ganzi Tibetan Autonomous Prefecture,Kangding 626000,China)

机构地区：[1]西南民族大学畜牧兽医学院,成都610041 [2]四川省兽药监察所,成都610041 [3]四川省甘孜藏族自治州畜牧业科学研究所,康定626000

出　　处：《动物营养学报》2025年第2期1340-1353,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：四川省重点研发项目(2023YFN0044);四川省肉羊创新团队(SCCXTD-2024-14);西南民族大学科研资助项目(2023KYZZ17N)。

摘　　要：本试验旨在研究1株羊源卷曲乳杆菌(Lactobacillus crispatus)DY38的体外益生特性及抗氧化活性,为进一步将其开发为羊用微生态制剂奠定基础。测定羊源卷曲乳杆菌DY38株的产酸能力、抑菌能力、耐酸耐胆盐能力、细胞黏附性能及抗氧化能力,从而评价其益生特性和抗氧化活性。结果显示:1)羊源卷曲乳杆菌DY38对羊源致病性大肠杆菌、肠炎沙门氏菌和产气荚膜梭菌等均有良好抑菌效果;2)羊源卷曲乳杆菌DY38在pH 2.0条件下的存活率为62.73%,在0.3%胆盐浓度下的存活率为66.85%,具有良好的胃肠道耐受力;3)羊源卷曲乳杆菌DY38对常用抗生素敏感,且无溶血性;4)羊源卷曲乳杆菌DY38的自聚集能力、共聚集能力和表面疏水性分别为64.83%、62.81%和80.69%;5)羊源卷曲乳杆菌DY38对Caco-2细胞的黏附能力较强,且无细胞裂解作用;6)羊源卷曲乳杆菌DY38的胞内提取物具有更强的超氧阴离子自由基清除能力,发酵上清液显示出更高的2,2-二苯基-1-苦酰肼(DPPH)自由基、羟自由基清除能力和还原能力。上述结果表明,羊源卷曲乳杆菌DY38具有良好的益生特性,具有进一步开发成为微生态制剂用于防治羊腹泻等疾病的潜在价值。This study aimed to investigate the in vitro probiotic properties and antioxidant activity of Lactobacillus crispatus DY38 from goats,providing a foundation for its development as a probiotic supplement for goats.The acid production ability,antibacterial ability,acid and bile salt tolerance,cell adhesion property,and antioxidant capacity of the Lactobacillus crispatus DY38 from goats were measured to evaluate its probiotic properties and antioxidant activity.The results showed as follows:1)Lactobacillus crispatus DY38 from goats exhibited strong inhibitory effects against pathogenic Escherichia coli,Salmonella enteritis and Clostridium perfringens isolated from goats;2)the survival rate of Lactobacillus crispatus DY38 from goats under pH 2.0 was 62.73%,and 66.85%in 0.3%bile salts,and it had good gastrointestinal tolerance;3)it was sensitive to commonly used antibiotics and showed no hemolytic activity;4)the auto-aggregation ability,co-aggregation ability and surface hydrophobicity of Lactobacillus crispatus DY38 from goats were 64.83%,62.81%and 80.69%,respectively;5)it exhibited strong adhesion to Caco-2 cells without cytolytic effect;6)the intracellular extract of Lactobacillus crispatus DY38 from goats demonstrated strong superoxide anion radical scavenging ability,and its fermentation supernatant exhibited high 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical and hydroxyl radical scavenging ability,and high reducing capacity.These findings indicate that Lactobacillus crispatus DY38 from goats has good probiotic properties and potential for development as a probiotic supplement to prevent and treat diarrhea and other diseases in goats.

关 键 词：山羊 卷曲乳杆菌 益生特性 抗氧化 

分 类 号：S816[农业科学—饲料科学]