DNA walker induced“signal on”fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer  

作　　者：Gaojian Yang Zhiyang Li Rabia Usman Zhu Chen Yuan Liu Song Li Hui Chen Yan Deng Yile Fang Nongyue He 

机构地区：[1]State Key Laboratory of Digital Medical Engineering,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412007,China [3]Department of Clinical Laboratory,the Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,China [4]Institute of Cytology and Genetics,School of Basic Medical Sciences,Hengyang Medical School,University of South China,Hengyang 421001,China [5]Institute for Future Sciences,University of South China,Changsha 410000,China

出　　处：《Chinese Chemical Letters》2025年第2期300-303,共4页中国化学快报(英文版)

基　　金：supported financially by the National Natural Science Foundation of China(NSFC,Nos.62071119 and 62075098);the National Key Research and Development Program of China(Nos.2017YFA0205301 and 2018YFC1602905)。

摘　　要：The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer.Hence,there is an urgent requirement to create biosensors that are time-saving,simple,and easily scalable in order to accomplish rapid,sensitive,and quantitative detection of extracellular vesicles.In this study,we present a self-propelled DNA walker powered by endonuclease Nt.Bbv CI,which enables the development of a“signal on”sensing platform for the rapid and highly sensitive detection of extracellular vesicles.The DNA motor employed tracks made of streptavidin magnetic beads,which consisted of substrate strands labeled with fluorescein and motor strands locked by aptamers.The aptamer recognition of the target protein on extracellular vesicles unlocked the motor strand,initiating the DNA motor process.After replacing the optimal buffer solution containing the endonuclease Nt.BbvC I,the motor strands autonomously moved along the streptavidin magnetic beads track,continuously releasing fluorescent molecules and producing detectable fluorescence signals.Under optimal conditions,the detection range was from 2×10~4particles/mL to 2×10~9particles/mL,with a detection limit of 2.9×10~3particles/mL,demonstrating excellent selectivity.This method has demonstrated good selectivity in different tumorderived extracellular vesicles and performs well in complex biological samples.The ability to effectively analyze surface proteins of extracellular vesicles in a short period of time gives our DNA walker a tremendous potential for developing simple and cost-effective clinical diagnostic devices.

关 键 词：Extracellular vesicles APTAMER Streptavidin magnetic beads Nt.BbvCI Detection 

分 类 号：O657.3[理学—分析化学] TP212[理学—化学] R735.2[自动化与计算机技术—检测技术与自动化装置]