LINC01772对A549细胞周期、凋亡及放疗敏感性的影响  

Effects of LINC01772 on cell cycle,apoptosis and radiosensitivity of A549

作　　者：何丽妃 朱琳 薛峰 闭祖悦 李赵鑫 HE Lifei;ZHU Lin;XUE Feng;BI Zuyue;LI Zhaoxin(Department of Oncology Radiotherapy,Affiliated Hospital of Guilin Medical Univer-sity,Guilin 541001,Guangxi,China;不详)

机构地区：[1]桂林医学院附属医院放射治疗科,广西桂林541001 [2]桂林医学院附属医院肿瘤内科,广西桂林541001

出　　处：《实用医学杂志》2025年第5期657-663,共7页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:82160471,82260498)。

摘　　要：目的探讨长链非编码RNA LINC01772对A549细胞周期、凋亡及放疗敏感性的影响。方法复苏及培养A549细胞,运用CCK-8、流式细胞术探究LINC01772敲减后A549细胞的细胞活力、细胞周期、细胞凋亡情况,Western blot检测凋亡相关蛋白。CCK-8、克隆形成实验测不同剂量放射线处理后A549细胞对放疗敏感性的改变,流式细胞术检查放疗后A549细胞的细胞周期和凋亡的情况。结果通过慢病毒转染敲低A549细胞中LINC01772后,A549细胞的细胞活力降低(P<0.01),凋亡增多(P<0.01),Bad、Cleaved Caspase-3的表达显著升高(P<0.01),而Bcl-2的表达显著降低(P<0.01);不同剂量放射线处理后,A549细胞的细胞活力下降且呈现剂量依赖(P<0.01),克隆形成能力显著减弱(P<0.01),细胞周期阻滞在G2/M期(P<0.01),细胞凋亡数量增多(P<0.01)。结论抑制长链非编码RNA LINC01772的表达,可显著抑制A549细胞活力、促进其凋亡、阻止其周期进程,并提高A549细胞放疗敏感性,该非编码RNA有可能成为肺癌放疗增敏的潜在靶点。Objective To explore the effects of long non-coding RNA LINC01772 on cell cycle,apoptosis and radiotherapy sensitivity of A549.Methods A549 cells were resuscitated and cultured for experimental purpose.The effects of LINC01772 knockdown on cell viability,cell cycle progression,and apoptosis were assessed using CCK-8 assays and flow cytometry,and apoptosis-related proteins were analyzed using western blotting.CCK-8 and clonogenic assays were used to measure the changes in the sensitivity of A549 cells to radiotherapy following various radiation doses,and flow cytometry to examine alterations in the cell cycle and apoptosis post-radiation treatment.Results Following lentiviral transfection that reduced LINC01772 levels in A549 cells,a significant decrease in cell viability(P<0.01)was observed alongside an increase in apoptosis(P<0.01).Notably,expressions of Bad and Cleaved Caspase-3 were significantly elevated(P<0.01),while Bcl-2 expression was markedly decreased(P<0.01).After exposure to different radiation doses,a dose-dependent reduction in A549 cell viability was noted(P<0.01),accompanied by a significant impairment of clonogenic ability(P<0.01),G2/M phase arrest within the cell cycle(P<0.01),and an increased incidence of apoptosis(P<0.01).Conclusion Inhibiting long non-coding RNA LINC01772 expression substantially diminishes cellular viability in A549 cells,enhances apoptotic processes,disrupts their cycling mechanisms,and augments their sensitivity to radiotherapy,suggesting that this non-coding RNA may serve as a potential target for enhancing lung cancer radiotherapy.

关 键 词：非小细胞肺癌 放射治疗 放疗敏感性 长链非编码RNA 

分 类 号：R734.2[医药卫生—肿瘤]